40S ribosomal protein S18 is a novel maternal peptidoglycan-binding protein that protects embryos of zebrafish from bacterial infections.

Abstract	Previous studies have shown that ribosomal proteins play important roles in ribosome assembly and protein translation, but other biological functions remain ill-defined. Here it is clearly demonstrated that RPS18 is a newly identified PGN-binding protein which is present abundantly in the eggs/embryos of zebrafish. Recombinant RPS18 not only identifies the bacterial signature molecule PGN, LPS, and LTA, and binds the bacteria as a pattern recognition receptor, but also kills the Gram-positive and Gram-negative bacteria as an antibacterial effector molecule. What is important is that, we reveal that microinjection of rRPS18 into early embryos significantly improved the resistance of the embryos against pathogenic Aeromonas hydrophila challenge, and co-injection of anti-RPS18 antibody could markedly reduced this improved bacterial resistance. In summary, these results indicate that RPS18 is a maternal immune factor that can protect the early embryos of zebrafish against pathogenic attacks. This work also provides another angle for understanding the biological functions of ribosomal proteins.
Authors	Xia Wang, Yiqing Ren, Chengming Gong, Yufei Chen, Xiaoping Ge, Jun Kong, Wenjing Sun, Xiaoyuan Du
Journal	Developmental and comparative immunology (Dev Comp Immunol) Vol. 125 Pg. 104212 (12 2021) ISSN: 1879-0089 [Electronic] United States
PMID	34310970 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright	Copyright © 2021. Published by Elsevier Ltd.
Chemical References	Anti-Bacterial Agents Carrier Proteins Lipopolysaccharides Peptidoglycan Receptors, Pattern Recognition Ribosomal Proteins Zebrafish Proteins ribosomal protein S18
Topics	Aeromonas hydrophila (immunology) Amino Acid Sequence Animals Anti-Bacterial Agents (metabolism) Bacteria (metabolism) Bacterial Infections (metabolism) Carrier Proteins (metabolism) Embryo, Nonmammalian (metabolism) Gram-Negative Bacteria Gram-Positive Bacteria Lipopolysaccharides (metabolism) Peptidoglycan (metabolism) Phagocytosis Receptors, Pattern Recognition (metabolism) Ribosomal Proteins (metabolism) Zebrafish (immunology) Zebrafish Proteins (metabolism)

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