Long noncoding RNAs (lncRNAs) and their crosstalks with other RNAs have been revealed to be closely related to
tumorigenesis and development, but their role in invasive
pituitary adenoma (IPA) remains largely unclear. In our study, LINC00473 was identified as the most upregulated
lncRNA in IPA by whole transcriptome
RNA sequencing (
RNA-Seq). Further, its related signaling pathway LINC00473/miR-502-3p/KMT5A was obtained by constructing a
competing endogenous RNA (
ceRNA) regulatory network. Their expression in IPA and non-invasive
pituitary adenoma (NIPA) tissues was verified by qRT-PCR. Then the effects and mechanisms of LINC00473 and its
ceRNA network on the proliferation of
pituitary adenoma (PA) cells were confirmed by gene overexpression or silencing techniques combined with
CCK-8 assay, EdU staining, flow cytometry assay, and double
luciferase reporter gene assay in PA cell lines AtT-20 and GT1-1 in vitro and in a xenograft model in vivo. LINC00473 is overexpressed in IPA and can promote PA cells proliferation. Mechanistically, overexpression of LINC00473 restricts miR-502-3p through the
ceRNA mechanism, upregulates KMT5A expression, and promotes the expression of
cyclin D1 and CDK2, which is conducive to the cell cycle process, thereby promoting the proliferation of PA cells, involving IPA progression.