Abstract | BACKGROUND: METHODS: RBCs of two ChAc patients during and after dasatinib treatment were characterized by the ESR, clinical hematology parameters and the 3D shape classification in stasis based on an artificial neural network. Furthermore, mathematical modeling was performed to understand the contribution of cell morphology and cell rigidity to the ESR. Microfluidic measurements were used to compare the RBC rigidity between ChAc patients and healthy controls. RESULTS: The mechano-morphological characterization of RBCs from two ChAc patients in an off-label treatment with dasatinib revealed differences in the ESR and the acanthocyte count during and after the treatment period, which could not directly be related to each other. Clinical hematology parameters were in the normal range. Mathematical modeling indicated that RBC rigidity is more important for delayed ESR than cell shape. Microfluidic experiments confirmed a higher rigidity in the normocytes of ChAc patients compared to healthy controls. CONCLUSIONS: The results increase our understanding of the role of acanthocytes and their associated properties in the ESR, but the data are too sparse to answer the question of whether the ESR is a suitable biomarker for treatment success, whereas a correlation between hematological and neuronal phenotype is still subject to verification.
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Authors | Antonia Rabe, Alexander Kihm, Alexis Darras, Kevin Peikert, Greta Simionato, Anil Kumar Dasanna, Hannes Glaß, Jürgen Geisel, Stephan Quint, Adrian Danek, Christian Wagner, Dmitry A Fedosov, Andreas Hermann, Lars Kaestner |
Journal | Biomolecules
(Biomolecules)
Vol. 11
Issue 5
(05 12 2021)
ISSN: 2218-273X [Electronic] Switzerland |
PMID | 34066168
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers
- Protein Kinase Inhibitors
- Dasatinib
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Topics |
- Acanthocytes
(drug effects, pathology)
- Adult
- Biomarkers
(blood)
- Blood Sedimentation
(drug effects)
- Cell Shape
(drug effects)
- Dasatinib
(therapeutic use)
- Erythrocytes
(drug effects)
- Humans
- Male
- Neuroacanthocytosis
(blood, drug therapy, pathology)
- Off-Label Use
- Protein Kinase Inhibitors
(therapeutic use)
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