Abstract |
Since chemotherapy drugs have dose-related side effects, there is still a need for finding new agents with suitable cytotoxic effects without any harmful effects. For this purpose, we evaluated the cytotoxic effects of Britannin that is a Sesquiterpene Lactone compound Inula aucheriana, alone or in combination with Vincristine (VCR), on Acute Lymphoblastic Leukemia (ALL)-derived MOLT-4 cells. In this study, we found that Britannin decreased the viability of MOLT-4 cells with the IC50 Values of 2 µM, but had no cytotoxic effects on normal cells or Peripheral Blood Mononuclear Cells (PBMCs). Our results also showed that Britannin decreased the proliferation of MOLT-4 cells by preventing the transition of the cells from the S phase of the cell cycle through the up-regulation of p21 and p27. Moreover, this agent induced ROS-mediated apoptosis by altering the expression of Bax, Bim, Caspase3, Bcl2, and XIAP. Britannin also produced a synergistic effect with Vincristine in MOLT-4 cells. Taken together, the results of this study showed for the first time that Britannin, as a natural Sesquiterpene Lactone, has cytotoxic effects that could be considered as an anti-leukemic agent in the treatment of ALL. However, there is still a demand for further studies that examine the efficacy and the safety of this purified compound.
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Authors | Hassan Mohammadlou, Maryam Hamzeloo-Moghadam, Amir Yami, Fatemeh Feizi, Marzieh Moeinifard, Ahmad Gharehbaghian |
Journal | Nutrition and cancer
(Nutr Cancer)
Vol. 74
Issue 3
Pg. 965-977
( 2022)
ISSN: 1532-7914 [Electronic] United States |
PMID | 34060394
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Lactones
- Phytochemicals
- Sesquiterpenes
- britannin
- Vincristine
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Topics |
- Antineoplastic Agents
(pharmacology)
- Apoptosis
- Cell Line, Tumor
- Humans
- Inula
- Lactones
(pharmacology)
- Leukocytes, Mononuclear
- Phytochemicals
(pharmacology)
- Precursor Cell Lymphoblastic Leukemia-Lymphoma
(drug therapy, metabolism)
- Sesquiterpenes
(pharmacology, therapeutic use)
- Vincristine
(pharmacology, therapeutic use)
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