Use of scREAD to explore and analyze single-cell and single-nucleus RNA-seq data for Alzheimer's disease.

Abstract

Single-cell RNA-sequencing (scRNA-seq) and single-nucleus RNA-sequencing (snRNA-seq) studies have provided remarkable insights into understanding the molecular pathogenesis of Alzheimer's disease. We recently developed scREAD, a database to provide comprehensive analyses of all the existing AD scRNA-seq and snRNA-seq data from the public domain. Here, we report protocols for using the scREAD web interface and running the backend workflow locally. Our protocols enable custom analyses of AD single-cell and single-nucleus gene expression profiles. For complete details on the use and execution of this protocol, please refer to Jiang et al. (2020).

• Authors: Cankun Wang, Yujia Xiang, Hongjun Fu, Qin Ma
• Journal: STAR protocols (STAR Protoc) Vol. 2 Issue 2 Pg. 100513 (06 18 2021) ISSN: 2666-1667 [Electronic] United States
• PMID: 34013209 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
• Copyright: © 2021 The Author(s).

Topics

• Alzheimer Disease (genetics, metabolism)
• Humans
• RNA-Seq
• Single-Cell Analysis