Autotransfusion refers to a
blood transfusion method in which the blood or blood components of the patient are collected under certain conditions, returned to himself when the patient needs surgery or emergency after a series of storing and processing. Although
autotransfusion can avoid
blood-borne diseases and adverse reactions related to allogeneic
blood transfusion, a series of structural and functional changes of erythrocytes will occur during extension of storage time, thus affecting the efficacy of clinical
blood transfusion. Our research was aimed to explore the change of erythrocyte
oxygen-carrying capacity in different storage time, such as effective
oxygen uptake (Q), P50,
2,3-DPG, Na+-K+-
ATPase, to detect membrane potential, the change of Ca2+, and
reactive oxygen species (ROS) change of erythrocytes. At the same time, Western blot was used to detect the expression of Mitofusin 1 (Mfn1) and Mitofusin 2 (Mfn2)
proteins on the cytomembrane, from the perspective of oxidative stress to explore the function change of erythrocytes after different storage time. This study is expected to provide experimental data for further clarifying the functional status of erythrocytes with different preservation time in patients with
autotransfusion, achieving accurate infusion of erythrocytes and improving the
therapeutic effect of
autologous blood transfusion, which has important clinical application value.