Abstract | BACKGROUND: METHODS: Thirty adult male New Zealand rabbits were randomly divided into five groups (n=6): a sham operation group ( Sham group), an operation without fluid therapy group ( Shock group), a BRS group, an ARS group, and a normal saline group (Saline group). The New Zealand rabbits experienced rapid bloodletting to shock status and maintained for 20 minutes except Sham group. The status of shock was maintained in the Shock group. The fluid was infused at a rate of 60 mL/kg per hour for 1.5 hours in three fluid therapy group. Measurement of vital signs, arterial blood gas tests, blood biochemistry, hematoxylin and eosin (HE) staining of lung tissue, TUNEL staining of the liver and kidney tissues, and analysis of intestinal flora were performed. RESULTS: The reduction in both base excess (BE) and bicarbonate ion (HCO3 -) caused by acidosis in rabbits with hemorrhagic shock was significantly improved in the BRS group when compared with the Saline group at infusion for 30 minutes (T3) and 30 minutes after infusion (T5) (BRS group vs. Saline group, BE: at T3, -4.83±3.60 vs. -12.50±3.27 mmol/L, P<0.01; at T5, -3.67±4.37 vs. -11.00±2.76 mmol/L, P<0.01; HCO3 -: at T3, 22.15±2.63 vs. 15.42±3.03 mmol/L, P<0.01; at T5, 23.15±2.9 vs. 16.23±3.07 mmol/L, P<0.01). Compared with Shock group, liver cell apoptosis due to hemorrhagic shock was relieved in both the BRS group and ARS group (BRS group vs. Shock group: 19.1±3.3 vs. 28.1±6.1, P<0.05; ARS group vs. Shock group: 19.8±5.4 vs. 28.1±6.1, P<0.05). CONCLUSIONS:
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Authors | Long Wang, Jingsheng Lou, Jiangbei Cao, Tao Wang, Jing Liu, Weidong Mi |
Journal | Annals of translational medicine
(Ann Transl Med)
Vol. 9
Issue 6
Pg. 462
(Mar 2021)
ISSN: 2305-5839 [Print] China |
PMID | 33850859
(Publication Type: Journal Article)
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Copyright | 2021 Annals of Translational Medicine. All rights reserved. |