Enzyme-Digested Peptides Derived from Lates calcarifer Enhance Wound Healing after Surgical Incision in a Murine Model.

Abstract

Surgical wounds are common injuries of skin and tissues and usually become a clinical problem. Until now, various synthetic and natural peptides have been widely explored as potential drug candidates for wound healing. Inhibition of the TNF-α signaling pathway and promotion of angiogenesis are suggested to be involved in their effects. Angiogenesis at the wound site is one of the essential requisites for rapid healing. In the present study, a novel peptide extract derived from the natural source Lates calcarifer, commonly known as sea bass or barramundi, was evaluated for its wound healing property. The specific acidic and enzymatic approaches were employed for producing sea bass extract containing small size peptides (molecular weight ranging from 1 kD to 5 kD). The cytotoxicity of the extract was examined in HaCaT and NIH3T3. After this, the effects of enzyme digested peptide extracts of sea bass on wound healing in mice were investigated. The peptide extracts (660 and 1320 mg/kg/day) and control protein (1320 mg/kg/day) was orally given to the wounded mice, respectively, for 12 days. The surgical method was improved by implanting a silicone ring at the wound site. The ring avoided the contracting effect in murine wounds, making it more closely related to a clinical condition. The results showed promising improvement at the wound site in mice. Sea bass peptide extracts accelerated the wound healing process and enhanced the microvessel formation at the wound site. The remarkable effects of this novel sea bass peptide extract in healing traumatic injuries revealed a new option for developing wound management.

• Authors: Yen-An Lin, Pei-Yi Chu, Wen-Lung Ma, Wei-Chung Cheng, Shu-Ting Chan, Juan-Cheng Yang, Yang-Chang Wu
• Journal: Marine drugs (Mar Drugs) Vol. 19 Issue 3 (Mar 16 2021) ISSN: 1660-3397 [Electronic] Switzerland
• PMID: 33809638 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Enzymes
• Peptides
• Tissue Extracts
• Tumor Necrosis Factor-alpha

Topics

• Animals
• Bass (metabolism)
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Enzymes (metabolism)
• HaCaT Cells
• Humans
• Mice
• Mice, Inbred C57BL
• NIH 3T3 Cells
• Peptides (isolation & purification, metabolism, pharmacology)
• Surgical Wound (drug therapy, pathology)
• Tissue Extracts (isolation & purification, metabolism, pharmacology)
• Tumor Necrosis Factor-alpha (antagonists & inhibitors)
• Wound Healing (drug effects)