Abstract | BACKGROUND: Neonatal encephalopathy often leads to lifelong disabilities with limited treatments currently available. The brain vasculature is an important factor in many neonatal neurological disorders but there is a lack of diagnostic tools to evaluate the brain vascular dysfunction of neonates in the clinical setting. Measurement of blood-brain barrier tight-junction (TJ) proteins have shown promise as biomarkers for brain injury in the adult. Here we tested the biomarker potential of tight-junctions in the context of neonatal brain injury. METHODS: The levels of TJ- proteins (occluding, claudin-5, and zonula occludens protein 1) in both blood plasma and cerebrospinal fluid (CSF) as well as blood-brain barrier function via 14C-sucrose (342 Da) and Evans blue extravasation were measured in a hypoxia/ischemia brain-injury model in neonatal rats. RESULTS: CONCLUSIONS:
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Authors | E Axel Andersson, Carina Mallard, C Joakim Ek |
Journal | Fluids and barriers of the CNS
(Fluids Barriers CNS)
Vol. 18
Issue 1
Pg. 7
(Feb 10 2021)
ISSN: 2045-8118 [Electronic] England |
PMID | 33568200
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers
- Claudin-5
- Occludin
- Zonula Occludens-1 Protein
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Topics |
- Animals
- Biomarkers
(blood, cerebrospinal fluid, metabolism)
- Blood-Brain Barrier
(metabolism)
- Claudin-5
(blood, cerebrospinal fluid, metabolism)
- Disease Models, Animal
- Female
- Hypoxia-Ischemia, Brain
(blood, cerebrospinal fluid, metabolism)
- Male
- Occludin
(blood, cerebrospinal fluid, metabolism)
- Rats
- Rats, Wistar
- Zonula Occludens-1 Protein
(blood, cerebrospinal fluid, metabolism)
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