Exploring the dysregulated mRNAs-miRNAs-lncRNAs interactions associated to idiopathic non-obstructive azoospermia.

Abstract

Non-obstructive azoospermia (NOA) is the most clinical problem in case of infertility. About 70% of NOA patients are idiopathic with uncharacterized molecular mechanisms. This study aimed to analyze the possible pathogenic miRNA-target gene interaction and lncRNA-miRNA association involved in NOA. In the current study, differentially expressed (DE) nRNAs, miRNAs and lncRNAs were determined using the microarray dataset and statistical software R. miRNAs-mRNA and miRNA-lncRNA interactions were identified and the base-pair binding between the seed region of miRNAs and complementary nucleotides in 3' UTR of mRNAs were analyzed. The influence of the validated single nucleotide polymorphisms (SNPs) was described by calculating the minimum free energy (MFE) of the interaction. A total of 74 mRNAs, 14 miRNAs, and 10 lncRNAs were identified to have significant differential expression in testicular tissue between patients and the fertile group. Four of the DE-mRNAs and all of the reported DE-miRNAs were upregulated. In addition, all of the represented DE-lncRNAs were showed to be downregulated. miR-509-5p and miR-27b-3p were found to interact with target gene polo-like kinase 1 (PLK1) and Cysteine-rich secretory protein2 (CRISP2), respectively. Rs550967205 (A > G) positioned at 3' UTR CRISP2 and rs544604911 (T > C) located at 3' UTR PLK1, with lowest MFE in miRNA-mRNA interaction, were assumed to have possible pathogenic roles linked to spermatogenesis arrest. The results of the study provide new clues to understand the regulatory roles of miRNAs and lncRNAs in the pathogenesis and diagnosis of idiopathic azoospermia. Communicated by Ramaswamy H. Sarma.

• Authors: Soudabeh Sabetian, Mahboubeh Zarei, Bahia Namavar Jahromi, Mohammad Hossein Morowvat, Seyed Mohammad Bagher Tabei, Claudia Cava
• Journal: Journal of biomolecular structure & dynamics (J Biomol Struct Dyn) Vol. 40 Issue 13 Pg. 5956-5964 (08 2022) ISSN: 1538-0254 [Electronic] England
• PMID: 33499760 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 3' Untranslated Regions
• CRISP2 protein, human
• Cell Adhesion Molecules
• MicroRNAs
• RNA, Long Noncoding
• RNA, Messenger

Topics

• 3' Untranslated Regions (genetics)
• Azoospermia (diagnosis, genetics)
• Cell Adhesion Molecules
• Gene Regulatory Networks
• Humans
• Male
• MicroRNAs (genetics, metabolism)
• RNA, Long Noncoding (genetics, metabolism)
• RNA, Messenger (genetics, metabolism)