Abstract |
Drug repurposing is a feasible strategy for the development of novel therapeutic applications. However, its potential use for oral treatments and impact on host microbiota remain underexplored. Here, we assessed the influences of topical oral applications of a repurposed FDA-approved drug, thonzonium bromide, on gastrointestinal microbiomes and host tissues in a rat model of dental caries designed to reduce cross-contamination associated with coprophagy. Using this model, we recapitulated the body site microbiota that mirrored the human microbiome profile. Oral microbiota was perturbed by the treatments with specific disruption of Rothia and Veillonella without affecting the global composition of the fecal microbiome. However, disturbances in the oral-gut microbial interactions were identified using nestedness and machine learning, showing increased sharing of oral taxon Sutterella in the gut microbiota. Host-tissue analyses revealed caries reduction on teeth by thonzonium bromide without cytotoxic effects, indicating bioactivity and biocompatibility when used orally. Altogether, we demonstrate how an oral treatment using a repurposed drug causes localized microbial disturbances and therapeutic effects while promoting turnover of specific oral species in the lower gut in vivo.
|
Authors | Aurea Simon-Soro, Dongyeop Kim, Yong Li, Yuan Liu, Tatsuro Ito, Kenneth R Sims Jr, Danielle S W Benoit, Kyle Bittinger, Hyun Koo |
Journal | NPJ biofilms and microbiomes
(NPJ Biofilms Microbiomes)
Vol. 7
Issue 1
Pg. 7
(01 22 2021)
ISSN: 2055-5008 [Electronic] United States |
PMID | 33483519
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
|
Chemical References |
- Pyrimidines
- Quaternary Ammonium Compounds
- thonzonium bromide
|
Topics |
- Animals
- Bacteria
(classification, drug effects, isolation & purification)
- Dental Caries
(drug therapy, microbiology)
- Disease Models, Animal
- Drug Repositioning
- Feces
(microbiology)
- Gastrointestinal Microbiome
(drug effects)
- Humans
- Microbiota
(drug effects)
- Mouth
(microbiology)
- Pyrimidines
(pharmacology, therapeutic use)
- Quaternary Ammonium Compounds
(pharmacology, therapeutic use)
- Rats
|