Beneficial Effects of a Mixture of Algae and Extra Virgin Olive Oils on the Age-Induced Alterations of Rodent Skeletal Muscle: Role of HDAC-4.

Abstract

Aging is associated with a progressive decline in skeletal muscle mass, strength and function (sarcopenia). We have investigated whether a mixture of algae oil (25%) and extra virgin olive oil (75%) could exert beneficial effects on sarcopenia. Young (3 months) and old (24 months) male Wistar rats were treated with vehicle or with the oil mixture (OM) (2.5 mL/kg) for 21 days. Aging decreased gastrocnemius weight, total protein, and myosin heavy chain mRNA. Treatment with the OM prevented these effects. Concomitantly, OM administration decreased the inflammatory state in muscle; it prevented the increase of pro-inflammatory interleukin-6 (IL-6) and the decrease in anti-inflammatory interleukin-10 (IL-10) in aged rats. The OM was not able to prevent aging-induced alterations in either the insulin-like growth factor I/protein kinase B (IGF-I/Akt) pathway or in the increased expression of atrogenes in the gastrocnemius. However, the OM prevented decreased autophagy activity (ratio protein 1A/1B-light chain 3 (LC3b) II/I) induced by aging and increased expression of factors related with muscle senescence such as histone deacetylase 4 (HDAC-4), myogenin, and IGF-I binding protein 5 (IGFBP-5). These data suggest that the beneficial effects of the OM on muscle can be secondary to its anti-inflammatory effect and to the normalization of HDAC-4 and myogenin levels, making this treatment an alternative therapeutic tool for sarcopenia.

• Authors: Daniel González-Hedström, Teresa Priego, Asunción López-Calderón, Sara Amor, María de la Fuente-Fernández, Antonio Manuel Inarejos-García, Ángel Luis García-Villalón, Ana Isabel Martín, Miriam Granado
• Journal: Nutrients (Nutrients) Vol. 13 Issue 1 (Dec 25 2020) ISSN: 2072-6643 [Electronic] Switzerland
• PMID: 33375628 (Publication Type: Journal Article)

Chemical References

• Fatty Acids, Omega-3
• Muscle Proteins
• Myogenin
• Oils
• Olive Oil
• RNA, Messenger
• Insulin-Like Growth Factor I
• HDAC4 protein, rat
• Histone Deacetylases
• Myosin Heavy Chains

Topics

• Aging (physiology)
• Animals
• Fatty Acids, Omega-3 (administration & dosage)
• Histone Deacetylases (analysis, physiology)
• Inflammation (prevention & control)
• Insulin-Like Growth Factor I (analysis, genetics)
• Male
• Muscle Proteins (analysis)
• Muscle, Skeletal (chemistry, drug effects, physiology)
• Myogenin (analysis)
• Myosin Heavy Chains (genetics)
• Oils (administration & dosage)
• Olive Oil (administration & dosage)
• Organ Size (drug effects)
• RNA, Messenger (analysis)
• Rats
• Rats, Wistar
• Sarcopenia (prevention & control)
• Stramenopiles