Jiaolong capsule protects SD rats against 2,4,6-trinitrobenzene sulfonic acid induced colitis.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Jiaolong capsule (JLC) was approved for the therapy of gastrointestinal diseases by the State Food and Drug Administration (SFDA) of China. It has a satisfactory curative effect in the treatment of patients with inflammatory bowel disease, however, the mechanism remains to be elucidated.

AIM OF THE STUDY:

In current study, the effects and possible mechanisms of JLC on 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis were investigated.

MATERIALS AND METHODS:

Sulfasalazine and JLC were administrated orally and initialized 6 h after TNBS enema, once a day for seven consecutive days. The effect of JLC on intestinal microbial populations and LPS/TLR-4/NF-κB pathway was observed and assessed. Thirty female SD rats were distributed into six groups randomly and equally, namely, control, TNBS, TNBS + sulfasalazine (625 mg/kg), and TNBS + three different doses of JLC (25, 50, and 100 mg/kg) groups.

RESULTS:

The effect of JLC on restoring normal structures of colorectum and repairing colonic damage were superior to that of sulfasalazine. JLC showed a positive effect in re-balancing intestinal bacteria population of colitis, and suppressed the activation of LPS/TLR-4/NF-κB pathway.

CONCLUSION:

The results suggest that JLC demonstrated a beneficial effect on treating colitis in a rat model. The possible mechanisms may be through the regulatory effect of intestinal commensal bacteria and down-regulation of LPS/TLR-4/NF-κB pathway.

• Authors: Yuhua Li, Yang Sun, Fanrong Diao, Yiming Ruan, Gui'e Chen, Tianle Tang, Yongsheng Liu, Huiping Zhou, Wenming Lin, Mingzhi Dong, Tieming Liu, Qibing Mei, De Cai
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 269 Pg. 113716 (Apr 06 2021) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 33352238 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Elsevier B.V. All rights reserved.

Chemical References

• Drugs, Chinese Herbal
• Gastrointestinal Agents
• NF-kappa B
• Protective Agents
• Rela protein, rat
• Tlr4 protein, rat
• Toll-Like Receptor 4
• Transcription Factor RelA
• NF-KappaB Inhibitor alpha
• Sulfasalazine
• Trinitrobenzenesulfonic Acid
• Cyclooxygenase 2
• Ptgs2 protein, rat
• Dinoprostone
• Acetic Acid

Topics

• Acetic Acid (toxicity)
• Animals
• Behavior, Animal (drug effects)
• Colitis, Ulcerative (chemically induced, drug therapy)
• Colon (drug effects, pathology)
• Cyclooxygenase 2 (genetics, metabolism)
• Dinoprostone (metabolism)
• Disease Models, Animal
• Down-Regulation (drug effects)
• Drugs, Chinese Herbal (chemistry, pharmacology, therapeutic use)
• Female
• Gastrointestinal Agents (pharmacology, therapeutic use)
• Gastrointestinal Microbiome (drug effects)
• Mice, Inbred ICR
• NF-KappaB Inhibitor alpha (genetics, metabolism)
• NF-kappa B (genetics, metabolism)
• Pain (chemically induced, drug therapy)
• Protective Agents (chemistry, pharmacology, therapeutic use)
• Rats, Sprague-Dawley
• Signal Transduction (drug effects)
• Sulfasalazine (pharmacology, therapeutic use)
• Toll-Like Receptor 4 (biosynthesis, drug effects)
• Transcription Factor RelA (genetics, metabolism)
• Trinitrobenzenesulfonic Acid (toxicity)
• Mice
• Rats