Novel near-infrared fluorescence probe with large Stokes shift for monitoring CCl4-induced toxic hepatitis.

Abstract

Toxic hepatitis which is induced by chemical substance is a serious threat to human health. More and more studies have shown that peroxynitrite (ONOO-) is related with the development of toxic hepatitis. So it is important to find a tool to study ONOO- change during the diagnosis and therapy of toxic hepatitis. Herein, a series of novel near-infrared (NIR) fluorescence dyes (DDM-R) with long emission wavelength (740-770 nm) and large Stokes shift (~200 nm) are developed. Among the dyes, DDM-OH with great spectral performance and facilely modified feature is used to construct probe DDM-ONOO-. The probe have the preference of high sensitivity and excellent selectivity for ONOO-. In addition, DDM-ONOO- was applied in detecting exogenous and endogenous ONOO- in cells and further used in detecting ONOO- of CCl4-induced toxic hepatitis in cells by fluorescence imaging, 3D quantification analysis, flow cytometry. More importantly, by visualizing ONOO-, the probe was used to monitor the diagnosis of CCl4-induced toxic hepatitis in mice and evaluate the therapeutic efficacy of hepatoprotective medicines (NAC, SM, DDB). The results show that the probe will provide a powerful tool for the diagnosis and treatment of toxic hepatitis.

• Authors: Yang Tian, Dong-Ye Zhou, Wen-Li Jiang, Zun-Pan She, Yongfei Li, Chun-Yan Li
• Journal: Talanta (Talanta) Vol. 223 Issue Pt 1 Pg. 121720 (Feb 01 2021) ISSN: 1873-3573 [Electronic] Netherlands
• PMID: 33303166 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Elsevier B.V. All rights reserved.

Chemical References

• Fluorescent Dyes
• Peroxynitrous Acid

Topics

• Animals
• Chemical and Drug Induced Liver Injury (etiology)
• Fluorescent Dyes
• Mice
• Optical Imaging
• Peroxynitrous Acid