Nojirimycin suppresses inflammation via regulation of NF-κ B signaling pathways.

Abstract

Nojirimycin (NJ) is a compound in which the oxygen of the ring is replaced with an NH group in the D-glucose structure. NJ, which has a structure similar to D-glucose, is a powerful glucosidase inhibitor and an interesting compound. However, no anti-inflammatory effects of NJ have been reported. Therefore, to investigate its anti-inflammatory effect, the production and expression of inflammatory cytokines, as well as inflammatory mediators, such as iNOS and COX-2, were measured in LPS-stimulated RAW264.7 macrophages. In addition, the effects on the representative inflammatory signaling pathways, the suppression of NF-κ B, and the activation of MAPK were studied. The production of iNOS, COX-2, and inflammatory cytokines (PGE₂, IL-6, IL-1β, and TNF-α) after NJ treatment was significantly inhibited. In addition, NJ showed anti-inflammatory effects through suppression of LPS-induced NF-κ B activation. D-Glucose is structurally similar to NJ. The effects of these substances on RAW264.7 macrophages were evaluated. NJ reduced nitric oxide (NO) levels, whereas D-glucose had no significant effect. Overall, the results suggested that NJ is a potential anti-inflammatory compound.

• Authors: Su Bin Hyun, You Chul Chung, Chang -Gu Hyun
• Journal: Die Pharmazie (Pharmazie) Vol. 75 Issue 12 Pg. 637-641 (12 01 2020) ISSN: 0031-7144 [Print] Germany
• PMID: 33303056 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Cytokines
• Inflammation Mediators
• Lipopolysaccharides
• NF-kappa B
• 1-Deoxynojirimycin
• Nitric Oxide
• Nitric Oxide Synthase Type II
• Nos2 protein, mouse
• Cyclooxygenase 2
• nojirimycin

Topics

• 1-Deoxynojirimycin (analogs & derivatives, pharmacology)
• Animals
• Anti-Inflammatory Agents (pharmacology)
• Cell Survival (drug effects)
• Cyclooxygenase 2 (metabolism)
• Cytokines (drug effects, metabolism)
• Inflammation (drug therapy)
• Inflammation Mediators (metabolism)
• Lipopolysaccharides (pharmacology)
• MAP Kinase Signaling System (drug effects)
• Mice
• Molecular Structure
• NF-kappa B (metabolism)
• Nitric Oxide (biosynthesis, metabolism)
• Nitric Oxide Synthase Type II
• RAW 264.7 Cells
• Signal Transduction (drug effects)