The Protective Effects of Dexmedetomidine Preconditioning on Hepatic Ischemia/Reperfusion Injury in Rats.

Abstract

BACKGROUND:

Ischemia/reperfusion (IR) injury is 1 of the major problems in liver surgery. This study aims to evaluate the histologic and biochemical effects of dexmedetomidine on ischemia/reperfusion injury in the liver of rats.

METHODS:

Twenty-two Sprague-Dawley male rats were separated into 3 groups: group sham, IR (IR injury), and IR-D (IR with dexmedetomidine). Ischemia was induced for 45 minutes with portal clampage and the reperfusion period was 120 minutes. Group IR-D received 3 μg/kg of dexmedetomidine with loading for 10 minutes and then 3 μg/kg/h of dexmedetomidine was continuously injected intravenously 30 minutes before portal clampage. Biochemical factors (alanine aminotransferase and aspartate aminotransferase), variable cytokines (B cell lymphoma-2 (Bcl-2), Bax, caspase 3, caspase 8, nuclear factor-kappa B, interleukin (IL)-1β, IL-6, IL-10, mixed lineage kinase domain-like protein, and receptor-interacting protein kinase-3), and histologic findings were investigated.

RESULTS:

Dexmedetomidine preconditioning significantly suppressed the histologic damage. In the IR-D group, the expression of IL-6 was decreased and the Bcl-2 was increased when compared with the IR group.

CONCLUSION:

Dexmedetomidine suppresses hepatic IR injury and the protective mechanism appears to involve the decrease of IL-6 and upregulation of Bcl-2 expression, which result in the attenuation of inflammatory response and the inhibition of apoptosis.

• Authors: Hyunyoung Lim, Tae Yeon Kim, Soo Yeon Kim, Soo Jin Ro, Su Rim Koh, Sun Ryu, Justin Sangwook Ko, Mi Ae Jeong
• Journal: Transplantation proceedings (Transplant Proc) 2021 Jan-Feb Vol. 53 Issue 1 Pg. 427-435 ISSN: 1873-2623 [Electronic] United States
• PMID: 33280824 (Publication Type: Journal Article)
• Copyright: Copyright © 2020. Published by Elsevier Inc.

Chemical References

• Adrenergic alpha-2 Receptor Agonists
• Dexmedetomidine

Topics

• Adrenergic alpha-2 Receptor Agonists (pharmacology)
• Animals
• Apoptosis (drug effects)
• Dexmedetomidine (pharmacology)
• Liver (drug effects, pathology)
• Male
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (pathology)