Abstract | BACKGROUND: METHODS: This study recruited AD patients and used Aβ1-40 treated SH-SY5Y cells mimicking AD characteristics. The expression of miR-331-3p was estimated using reverse transcription quantitative PCR. A receiver operating characteristic (ROC) analysis was used to evaluate the diagnostic value of miR-331-3p, and the correlation of miR-331-3p with patients' Mini-Mental State Examination (MMSE) scores and serum proinflammatory cytokines were analyzed. The effects of miR-331-3p on neuronal viability and inflammatory response were explored in SH-SY5Y cells by in vitro analysis. RESULTS: In AD patients and Aβ1-40 treated SH-SY5Y cells, the expression of miR-331-3p was significantly downregulated. Serum miR-331-3p had certain diagnostic potential and was correlated with the MMSE scores and serum proinflammatory cytokine levels of AD patients. In Aβ1-40-treated SH-SY5Y cells, the overexpression of miR-331-3p enhanced cell viability and inhibited inflammatory responses. CONCLUSION: The data of this study indicated that serum expression of miR-331-3p is decreased in AD patients, and is correlated with the MMSE scores and proinflammatory cytokine levels of AD patients. In addition, miR-331-3p can regulate the cell viability and the expression of pro-inflammatory cytokines of Aβ1-40 treated SH-SY5Y cells, indicating the potential neuroprotective role of miR-331-3p.
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Authors | Qingling Liu, Chengbin Lei |
Journal | Experimental gerontology
(Exp Gerontol)
Vol. 144
Pg. 111187
(02 2021)
ISSN: 1873-6815 [Electronic] England |
PMID | 33279668
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2020 Elsevier Inc. All rights reserved. |
Chemical References |
- MIRN331 microRNA, human
- MicroRNAs
- Neuroprotective Agents
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Topics |
- Alzheimer Disease
(diagnosis, genetics)
- Cell Survival
- Humans
- MicroRNAs
(genetics)
- Neurodegenerative Diseases
- Neuroprotective Agents
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