Abstract | OBJECTIVE: MATERIALS AND METHODS: A total of 36 Sprague-Dawley (SD) rats weighing 180-200 g were randomly divided into sham operation group (n=12), model group (n=12), and miR-34a inhibitor group (n=12). Renal ischemia-reperfusion modeling was performed in rats of model group and miR-34a inhibitor group. Those in the sham operation group received the same procedures without ligation. 200 μL of miR-34a inhibitor was pre-injected before modeling in rats of miR-34a inhibitor group. An automatic biochemical analyzer was used to detect serum creatinine and urea nitrogen levels in each group of rats, thus reflecting renal functions. The expressions of B-cell lymphoma 2 (Bcl-2), an apoptotic protein, and KLF4, a transcription factor, were detected via Western blotting. Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) assay was conducted to measure the expression levels of miR-34a and KLF4 in rat renal tissues in each group. Immunohistochemistry was employed to determine the expressions of inflammatory factors tumor necrosis factor-alpha (TNF-α) and interleukin-10 (IL-10). Additionally, terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate- biotin nick end labeling (TUNEL) staining was utilized to determine the apoptotic rate in rat kidney tissues in each group. RESULTS: Compared with those in the sham operation group, serum creatinine level, urea nitrogen level, expressions of miR-34a, TNF-α and Bcl-2 (p<0.05) increased, while the levels of KLF4 and IL-10 (p<0.05) decreased in the model group. Apoptosis rate was also higher in the model group than the controls. In comparison with the model group, miR-34a inhibitor group had lowered serum creatinine level, urea nitrogen level, expressions of miR-34a, TNF-α and Bcl-2, and apoptotic rate (p<0.05), but raised levels of KLF4 and IL-10 (p<0.05), showing statistically significant differences. CONCLUSIONS: Downregulation of miR-34a ameliorates inflammatory response and apoptosis induced by renal ischemia-reperfusion by promoting KLF4 level, thus improving renal functions in rats.
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Authors | Y-Y Shi, Y-H Ma, R Zhang, R-S Li |
Journal | European review for medical and pharmacological sciences
(Eur Rev Med Pharmacol Sci)
Vol. 24
Issue 22
Pg. 11683-11689
(11 2020)
ISSN: 2284-0729 [Electronic] Italy |
PMID | 33275236
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Klf4 protein, rat
- Kruppel-Like Factor 4
- Kruppel-Like Transcription Factors
- MIRN34 microRNA, rat
- MicroRNAs
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Topics |
- Animals
- Apoptosis
- Disease Models, Animal
- Down-Regulation
- Inflammation
(metabolism, pathology)
- Kidney
(metabolism, pathology)
- Kruppel-Like Factor 4
- Kruppel-Like Transcription Factors
(genetics, metabolism)
- Male
- MicroRNAs
(genetics, metabolism)
- Rats
- Rats, Sprague-Dawley
- Reperfusion Injury
(metabolism, pathology)
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