Treponema pallidum in female sex workers from the Brazilian Marajó Archipelago: prevalence, risk factors, drug-resistant mutations and coinfections.

Abstract

BACKGROUND:

Female sex workers (FSWs) are an especially vulnerable group for syphilis and other sexually transmitted infection (STIs). This study determined the prevalence of syphilis in FSWs and factors associated with this disease in the Marajó Archipelago (northern Brazil), as well as the frequency of point mutations (A2058G and A2059G) in the 23S rRNA gene of Treponema pallidum and coinfections with hepatitis B virus (HBV), hepatitis C virus (HCV) and hepatitis D virus (HDV).

METHODS:

FSWs were diagnosed using a rapid qualitative test and the isolates were evaluated for the presence of point mutations by real-time PCR. Blood samples with T. pallidum were tested for the presence of HBV, HCV and HDV by ELISA and confirmed by real-time PCR. The factors associated with syphilis were identified using Poisson regression models.

RESULTS:

Overall, 41.1% FSWs tested positive for syphilis and 23.5% were infected with strains having A2058G/A2059G point mutations. HBV (23.0%) and HCV (8.1%) were detected among FSWs with syphilis. Six factors were associated with syphilis: low levels of education, reduced income, drug use, unprotected sex, a lengthy career in prostitution and a lack of regular medical check-ups.

CONCLUSIONS:

These findings indicate an urgent need for implementation of effective strategies to diagnose, prevent and treat syphilis, as well as other STIs, in this Brazilian region.

• Authors: Evelen C Coelho, Samara B Souza, Camila Carla S Costa, Luana M Costa, Luiz Marcelo L Pinheiro, Luiz Fernando A Machado, Gláucia C Silva-Oliveira, Luísa Caricio Martins, Paula Cristina R Frade, Aldemir B Oliveira-Filho
• Journal: Transactions of the Royal Society of Tropical Medicine and Hygiene (Trans R Soc Trop Med Hyg) Vol. 115 Issue 7 Pg. 792-800 (07 01 2021) ISSN: 1878-3503 [Electronic] England
• PMID: 33210137 (Publication Type: Journal Article, Research Support, N.I.H., Intramural)
• Copyright: © The Author(s) 2020. Published by Oxford University Press on behalf of Royal Society of Tropical Medicine and Hygiene.

Topics

• Brazil (epidemiology)
• Coinfection (epidemiology)
• Drug Resistance, Bacterial
• Female
• Humans
• Mutation
• Prevalence
• Risk Factors
• Sex Workers
• Syphilis (epidemiology)
• Treponema pallidum (drug effects, genetics)