Novel Therapeutic Effects of Pterosin B on Ang II-Induced Cardiomyocyte Hypertrophy.

Abstract

Pathological cardiac hypertrophy is characterized by an abnormal increase in cardiac muscle mass in the left ventricle, resulting in cardiac dysfunction. Although various therapeutic approaches are being continuously developed for heart failure, several studies have suggested natural compounds as novel potential strategies. Considering relevant compounds, we investigated a new role for Pterosin B for which the potential life-affecting biological and therapeutic effects on cardiomyocyte hypertrophy are not fully known. Thus, we investigated whether Pterosin B can regulate cardiomyocyte hypertrophy induced by angiotensin II (Ang II) using H9c2 cells. The antihypertrophic effect of Pterosin B was evaluated, and the results showed that it reduced hypertrophy-related gene expression, cell size, and protein synthesis. In addition, upon Ang II stimulation, Pterosin B attenuated the activation and expression of major receptors, Ang II type 1 receptor and a receptor for advanced glycation end products, by inhibiting the phosphorylation of PKC-ERK-NF-κB pathway signaling molecules. In addition, Pterosin B showed the ability to reduce excessive intracellular reactive oxygen species, critical mediators for cardiac hypertrophy upon Ang II exposure, by regulating the expression levels of NAD(P)H oxidase 2/4. Our results demonstrate the protective role of Pterosin B in cardiomyocyte hypertrophy, suggesting it is a potential therapeutic candidate.

• Authors: Chang Youn Lee, Han Ki Park, Bok-Sim Lee, Seongtae Jeong, Sung-Ae Hyun, Jung-Won Choi, Sang Woo Kim, Seahyoung Lee, Soyeon Lim, Ki-Chul Hwang
• Journal: Molecules (Basel, Switzerland) (Molecules) Vol. 25 Issue 22 (Nov 12 2020) ISSN: 1420-3049 [Electronic] Switzerland
• PMID: 33198253 (Publication Type: Journal Article)

Chemical References

• Ager protein, rat
• HMGB1 Protein
• Hbp1 protein, rat
• Indans
• NF-kappa B
• Reactive Oxygen Species
• Receptor for Advanced Glycation End Products
• Receptor, Angiotensin, Type 1
• pterosin B
• Angiotensin II

Topics

• Angiotensin II (chemistry)
• Animals
• Cell Line
• Cell Survival
• Cytosol (metabolism)
• HMGB1 Protein (metabolism)
• Heart (drug effects)
• Hypertrophy (drug therapy)
• Indans (pharmacology)
• Myocytes, Cardiac (drug effects, metabolism)
• NF-kappa B (metabolism)
• Rats
• Reactive Oxygen Species (metabolism)
• Receptor for Advanced Glycation End Products (metabolism)
• Receptor, Angiotensin, Type 1 (metabolism)
• Signal Transduction