Toluene diisocyanate-induced inflammation and airway remodeling involves autophagy in human bronchial epithelial cells.

Abstract

Toluene-diisocyanate (TDI) is one of the main causes of occupational asthma. To study the role of autophagy in TDI-induced airway inflammation and airway remodeling in bronchial airway epithelial (16HBE) cells. We treated 16HBE cells with TDI-human serum albumin (TDI-HSA) conjugate to observe reactive oxygen species (ROS) release, autophagy activation, airway inflammation and airway remodeling. 3-Methyladenine (3-MA) and Rapamycin (Rapa) intervention were used to explore the effects of autophagy on inflammatory response and protein expression related to airway remodeling in 16HBE cells treated with TDI-HSA. Experimental results suggested that various concentrations of TDI-HSA (0, 40, 80 and 120 μg/mL) increased the release of ROS and the expression of Nrf2, activated autophagy and increased the expression of AMPK, Beclin-1, LC3 and decreased the expression of p62, promoted the levels of IL-5, IL-6 and IL-8 in 16HBE cells. Results also showed that E-cadherin expression decreased but an increase was observed in α-SMA and MMP-9 in the TDI-HSA group. The treatment of TDI-HSA combined with Rapa aggravated the above reaction whereas the inverse was true for TDI-HSA combined with 3-MA. These results indicated that autophagy is involved in TDI-induced airway inflammation and airway remodeling as a positive regulatory mechanism, inhibiting autophagy can significantly alleviate the TDI-induced inflammatory response and attenuate airway remodeling protein expression in 16HBE cells.

• Authors: Bo Jiao, Yujun Chen, Yuting Yang, Linlin Sai, Gongchang Yu, Cunxiang Bo, Yu Zhang, Cheng Peng, Qiang Jia, Hua Shao
• Journal: Toxicology in vitro : an international journal published in association with BIBRA (Toxicol In Vitro) Vol. 70 Pg. 105040 (Feb 2021) ISSN: 1879-3177 [Electronic] England
• PMID: 33127434 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Elsevier Ltd. All rights reserved.

Chemical References

• Allergens
• Cytokines
• NF-E2-Related Factor 2
• NFE2L2 protein, human
• Reactive Oxygen Species
• Toluene 2,4-Diisocyanate
• Serum Albumin, Human

Topics

• Airway Remodeling (drug effects)
• Allergens (toxicity)
• Autophagy (drug effects)
• Bronchi (cytology)
• Cell Line
• Cell Survival (drug effects)
• Cytokines (metabolism)
• Epithelial Cells (drug effects, metabolism)
• Humans
• Inflammation (chemically induced, metabolism)
• NF-E2-Related Factor 2 (metabolism)
• Reactive Oxygen Species (metabolism)
• Serum Albumin, Human (toxicity)
• Toluene 2,4-Diisocyanate (toxicity)