Development of a U-bent plastic optical fiber biosensor with plasmonic labels for the detection of chikungunya non-structural protein 3.

Abstract

This study presents a novel plasmonic fiber optic sandwich immunobiosensor for the detection of chikungunya, an infectious mosquito-borne disease with chronic musculoskeletal pain and acute febrile illness, by exploiting non-structural protein 3 (CHIKV-nsP3) as a biomarker. A plasmonic sandwich immunoassay for CHIKV-nsP3 was realized on the surface of a compact U-bent plastic optical fiber (POF, 0.5 mm core diameter) with gold nanoparticles (AuNPs) as labels. The high evanescent wave absorbance (EWA) sensitivity of the U-bent probes allows the absorption of the light passing through the fiber by the AuNP labels, upon the formation of a sandwich immunocomplex of CHIKV-nsP3 on the core surface of the U-bent probe region. A simple optical set-up with a low-cost green LED and a photodetector on either end of the U-bent probe gave rise to a detection limit of 0.52 ng mL-1 (8.6 pM), and a linear range of 1-104 ng mL-1 with a sensitivity of 0.1043A530 nm/log(CnsP3). In addition, the plasmonic POF biosensor shows strong specificity towards the CHIKV-nsP3 analyte in comparison with Pf-HRP2, HIgG, and dengue whole virus. The results illustrate the potential of plasmonic POF biosensors for direct and sensitive point-of-care detection of the chikungunya viral disease.

• Authors: Ankitha George , M S Amrutha , Priyanshu Srivastava , Sujatha Sunil , V V R Sai , Ramanathan Srinivasan
• Journal: The Analyst (Analyst) Vol. 146 Issue 1 Pg. 244-252 (Jan 04 2021) ISSN: 1364-5528 [Electronic] England
• PMID: 33107522 (Publication Type: Journal Article)

Chemical References

• Plastics
• Gold

Topics

• Animals
• Biosensing Techniques
• Chikungunya Fever (diagnosis)
• Gold
• Metal Nanoparticles
• Optical Fibers
• Plastics