Abstract | OBJECTIVE: METHODS: The optimal working concentration of pingyangmycin (PYM) was detected by CCK-8 assay, and temperatures of 39, 42, and 45 ℃ were applied to the oral squamous cell carcinoma CAL27, SCC-15, and Tca8113 cell lines. The effects of different treatments on the apoptosis, calreticulin (CRT) membrane expression and high-mobility group box 1 ( HMGB1) secretion of the cells were detected by using Annexin V/ propidium iodide (PI), flow cytometry and enzyme-linked immunosorbent assay (ELISA) assay. SPSS 20.0 software was used for statistical analysis. RESULTS: Both hyperthermia and chemotherapy could increase the membrane expression of CRT and the secretion of HMGB1, and furthermore, thermo- chemotherapy group showed significantly increased in apoptosis, CRT membrane expression rate and HMGB1 secretion compared with chemotherapy group, and the difference was statistically significant (P<0.05). CONCLUSIONS:
|
Authors | Qiao-Zhen Sun, Fan Shi, Dan Luo, Ting Xu, Sheng-Zhi Wang |
Journal | Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology
(Hua Xi Kou Qiang Yi Xue Za Zhi)
Vol. 38
Issue 5
Pg. 502-508
(Oct 01 2020)
ISSN: 2618-0456 [Electronic] China |
PMID | 33085232
(Publication Type: Journal Article)
|
Chemical References |
|
Topics |
- Apoptosis
- Carcinoma, Squamous Cell
(therapy)
- Cell Line, Tumor
- HMGB1 Protein
- Humans
- Mouth Neoplasms
(therapy)
|