Abstract | BACKGROUND: We evaluated the diagnostic value of plasma Macrophage inhibitory cytokine-1 (MIC-1) in distinguishing patients with nasopharyngeal carcinoma (NPC) and explored its complementary role with widely used Epstein-Barr virus (EBV) related markers, EBV capsid antigen-specific IgA (VCA- IgA) and EBV copy number. METHODS: ELISA was used to analyze the plasma MIC-1 levels in 190 NPC patients, 72 VCA- IgA-positive healthy donors (VP), and 219 normal subjects with negative VCA- IgA (VN). 10 pairs of plasma samples before and after radiotherapy were also included. RESULTS: The plasma MIC-1 levels were significantly higher in NPC patients (Median: 678.39 ng/mL) than those in VN and VP (310.29 and 294.59, p < 0.001). Receiver operating characteristic (ROC) curves of the MIC-1 concentrations revealed that the area under the ROC curve (AUC) was 0.790 (95% confidence interval [CI]: 0.748-0.832), with a sensitivity of 63.7%, and a specificity of 85.9% respectively, for distinguishing NPC patients from the healthy donors. Similarly, between NPC and VP, ROC was 0.796 (0.738-0.853) with sensitivity of 63.7%, and specificity of 88.9%. In addition, between NPC and VN, ROC was 0.788(0.744-0.832) with sensitivity of 63.7%, and specificity of 84.9%. Further, we found that MIC-1 could complement VCA- IgA and EBV DNA markers, with a negative rate of 88.9% in VCA- IgA-positive healthy controls, and a positive rate of 59.0% in EBV DNA negative NPC patients, respectively. Also, the MIC-1 plasma concentration dropped significantly after radiotherapy (p = 0.027). CONCLUSIONS: MIC-1 can complement VCA- IgA titers and EBV DNA copy number tests in NPC detection, improve identification of EBV DNA-negative NPC patients, and distinguish NPC from VCA - IgA positive healthy controls.
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Authors | Shan Xing, Huilan Li, Yingqi Pi, Tao Zeng, Qi Huang, Guoping Ou, Ning Xue |
Journal | Technology in cancer research & treatment
(Technol Cancer Res Treat)
2020 Jan-Dec
Vol. 19
Pg. 1533033820956991
ISSN: 1533-0338 [Electronic] United States |
PMID | 33025864
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers
- GDF15 protein, human
- Growth Differentiation Factor 15
- Immunoglobulin A
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Topics |
- Adult
- Aged
- Biomarkers
- Disease Susceptibility
- Enzyme-Linked Immunosorbent Assay
- Epstein-Barr Virus Infections
(complications, virology)
- Female
- Growth Differentiation Factor 15
(blood)
- Herpesvirus 4, Human
- Humans
- Immunoglobulin A
(blood, immunology)
- Male
- Middle Aged
- Nasopharyngeal Carcinoma
(blood, diagnosis, etiology)
- Neoplasm Staging
- Prognosis
- Viral Load
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