Abstract | BACKGROUND: OBJECTIVE: METHOD: Fluorescence-activated cell sorting and adhesion assays were used to examine monocyte phenotype and ex vivo oxidized low-density lipoprotein uptake and adhesion in the patient before and after treatment with SPPT. Effects of plasma from the patient on the phenotype and adhesion of monocytes from a healthy participant were determined. RESULTS: SPPT improved hypercholesterolemia and cutaneous xanthomas. Before treatment, the patient had lower frequency of nonclassical monocytes but higher frequency of intermediate monocytes than the control participant. Before treatment, monocytes from the patient with LpX showed more intracellular lipid accumulation, alterations in several cell surface markers and intracellular cytokines, as well as enhanced oxidized low-density lipoprotein uptake and reduced adhesion compared with control. After SPPT, the phenotypes of monocytes from the patient with LpX were similar to control monocytes. Incubation with plasma from the patient before treatment as compared with plasma from the control participant or the patient after treatment increased CD11c expression and adhesion of monocytes from a healthy participant. CONCLUSION: LpX-induced hypercholesterolemia increased lipid accumulation and altered the phenotype of monocytes, which may contribute to cutaneous xanthoma development. Removal of LpX by SPPT reduced lipid accumulation and improved monocyte phenotype, likely contributing to xanthoma resolution.
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Authors | Zeqin Lian, Anum Saeed, Xueying Peng, Xiaoyuan Dai Perrard, Xiaoming Jia, Aliza Hussain, Christie M Ballantyne, Huaizhu Wu |
Journal | Journal of clinical lipidology
(J Clin Lipidol)
2020 Nov - Dec
Vol. 14
Issue 6
Pg. 850-858
ISSN: 1933-2874 [Print] United States |
PMID | 33011137
(Publication Type: Case Reports, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2020 National Lipid Association. Published by Elsevier Inc. All rights reserved. |
Chemical References |
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Topics |
- Humans
- Hypercholesterolemia
(blood, immunology)
- Lipoprotein-X
(blood)
- Monocytes
(cytology)
- Phenotype
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