Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: The Tibetan turnip (Brassica rapa L.) has a wide array of medicine properties including heat-clearing, detoxifying and anti- hypoxia as listed in the famous centuries-old Tibetan medicine classic "The Four Medical Tantras". Evidence-based medicine also indicated the anti-hypoxic effect of turnips, suggesting a potential link to neuroprotective effect on ischemic stroke. This thereby enables turnips to serve as a novel nontoxic agent in related treatment. AIM OF THE STUDY: MATERIALS AND METHODS: RESULTS:
AET can reduce cerebral infarct volume and ameliorate behavioral deficits of MCAO/R mice dose-dependently. In vitro experiment further demonstrated that suitable concentrations of AET inhibited ROS, LDH production and restored mitochondrial expression induced by OGD/R. AET pretreatment can reverse the OGD/R-induced decreased level of phosphorylation of PI3K, Akt, mTOR, whereas this effect was blocked in the LY294002 (PI3K inhibitor) treatment group. CONCLUSIONS:
AET improved the survival of OGD/R-injured HT-22 cells by activating the PI3K/Akt/mTOR pathway. Based on the results above, aqueous extract of turnip has a protective effect on focal cerebral ischemic injury.
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Authors | Hanyi Hua, Wenyi Zhang, Jiaying Li, Jiayi Li, Chang Liu, Yahui Guo, Yuliang Cheng, Fuwei Pi, Yunfei Xie, Weirong Yao, Yanqin Gao, He Qian |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 265
Pg. 113410
(Jan 30 2021)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 32980487
(Publication Type: Journal Article)
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Copyright | Copyright © 2020 Elsevier B.V. All rights reserved. |
Chemical References |
- Neuroprotective Agents
- Plant Extracts
- Reactive Oxygen Species
- mTOR protein, mouse
- Oncogene Protein v-akt
- TOR Serine-Threonine Kinases
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Topics |
- Animals
- Brain Ischemia
(drug therapy, pathology)
- Brassica rapa
(chemistry)
- Cell Line
- Disease Models, Animal
- Infarction, Middle Cerebral Artery
- Male
- Mice
- Mice, Inbred C57BL
- Neuroprotective Agents
(isolation & purification, pharmacology)
- Oncogene Protein v-akt
(metabolism)
- Phosphatidylinositol 3-Kinases
(metabolism)
- Plant Extracts
(pharmacology)
- Reactive Oxygen Species
(metabolism)
- Reperfusion Injury
(drug therapy, pathology)
- TOR Serine-Threonine Kinases
(metabolism)
- Tibet
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