Abstract |
The replication cycle and pathogenesis of the Plasmodium malarial parasite involves rapid expansion in red blood cells (RBCs), and variants of certain RBC-specific proteins protect against malaria in humans. In RBCs, bisphosphoglycerate mutase (BPGM) acts as a key allosteric regulator of hemoglobin/ oxyhemoglobin. We demonstrate here that a loss-of-function mutation in the murine Bpgm (BpgmL166P) gene confers protection against both Plasmodium-induced cerebral malaria and blood-stage malaria. The malaria protection seen in BpgmL166P mutant mice is associated with reduced blood parasitemia levels, milder clinical symptoms, and increased survival. The protective effect of BpgmL166P involves a dual mechanism that enhances the host's stress erythroid response to Plasmodium-driven RBC loss and simultaneously alters the intracellular milieu of the RBCs, including increased oxyhemoglobin and reduced energy metabolism, reducing Plasmodium maturation, and replication. Overall, our study highlights the importance of BPGM as a regulator of hemoglobin/ oxyhemoglobin in malaria pathogenesis and suggests a new potential malaria therapeutic target.
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Authors | Guoyue Xu, Rebekah van Bruggen, Christian O Gualtieri, Neda Moradin, Adrien Fois, Diane Vallerand, Mariana De Sa Tavares Russo, Angelia Bassenden, Wenyun Lu, Mifong Tam, Sylvie Lesage, Hélène Girouard, Daina Zofija Avizonis, Geneviève Deblois, Josef T Prchal, Mary Stevenson, Albert Berghuis, Tom Muir, Joshua Rabinowitz, Silvia M Vidal, Nassima Fodil, Philippe Gros |
Journal | Cell reports
(Cell Rep)
Vol. 32
Issue 12
Pg. 108170
(09 22 2020)
ISSN: 2211-1247 [Electronic] United States |
PMID | 32966787
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Bisphosphoglycerate Mutase
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Topics |
- Amino Acid Sequence
- Amino Acid Substitution
- Anemia
(etiology, prevention & control)
- Animals
- Base Sequence
- Bisphosphoglycerate Mutase
(chemistry, deficiency, genetics, metabolism)
- Enzyme Stability
- Erythrocytes
(enzymology, parasitology)
- Erythropoiesis
- Extracellular Matrix
(metabolism)
- Female
- HEK293 Cells
- Humans
- Malaria, Cerebral
(complications, enzymology, prevention & control)
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Mutant Strains
- Mutation
(genetics)
- Parasites
(growth & development)
- Plasmodium
(growth & development)
- Polycythemia
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