Abstract | BACKGROUND:
Mannitol increases blood-brain barrier permeability and can improve the efficiency of systemically administered stem cells by facilitating stem cell entry from the periphery into the injured brain. The aim of this study was to elucidate the neuroprotective effects of a combination of mannitol pretreatment and stem cell transplantation on stroke-induced neural injury. METHODS: RESULTS:
PKH-26 labeling revealed no difference in the number of stem cells that had migrated into the injured brain, and hADSC transplantation did not improve the infarct volume. However, neurobehavioral functions improved in the mannitol group. IHC showed higher numbers of RECA-1-positive cells in the peri-infarcted brain and BrdU-/DCX-colocalized cells in the subventricular zone in the mannitol group. IBA-1-positive cell number decreased in the hADSC-only and mannitol-pretreatment groups compared with the vehicle group even though there was no difference between the former two groups. CONCLUSION:
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Authors | Sang-Hoon Lee, Ho-Young Kang, Jong-Hoon Kim, Dong-Hyuk Park |
Journal | Tissue engineering and regenerative medicine
(Tissue Eng Regen Med)
Vol. 17
Issue 5
Pg. 695-704
(10 2020)
ISSN: 2212-5469 [Electronic] Korea (South) |
PMID | 32901436
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Dcx protein, rat
- Doublecortin Protein
- Mannitol
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Topics |
- Animals
- Cell Movement
- Disease Models, Animal
- Doublecortin Protein
- Infarction, Middle Cerebral Artery
(drug therapy)
- Mannitol
- Rats
- Stem Cell Transplantation
- Stroke
(drug therapy)
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