Amplification-free profiling of microRNA-122 biomarker in DILI patient serums, using the luminex MAGPIX system.

Abstract

Dynamic chemical labelling is a single-base specific method to enable detection and quantification of micro-Ribonucleic Acids in biological fluids without extraction and pre-amplification. In this study, dynamic chemical labelling was combined with the Luminex MAGPIX system to profile levels of microRNA-122 biomarker in serum from patients with Drug-Induced Liver Injury.

• Authors: Antonio Marín-Romero, Mavys Tabraue-Chávez, James W Dear, Rosario M Sánchez-Martín, Hugh Ilyine, Juan J Guardia-Monteagudo, Mario A Fara, Francisco J López-Delgado, Juan J Díaz-Mochón, Salvatore Pernagallo
• Journal: Talanta (Talanta) Vol. 219 Pg. 121265 (Nov 01 2020) ISSN: 1873-3573 [Electronic] Netherlands
• PMID: 32887156 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Elsevier B.V. All rights reserved.

Chemical References

• Biomarkers
• MIRN122 microRNA, human
• MicroRNAs

Topics

• Biomarkers
• Chemical and Drug Induced Liver Injury
• Humans
• MicroRNAs