Many parts of the plant Nyctanthes arbor-tristis Linn. are widely investigated for their
biological properties. Purified Arbortristosides from seeds are reported as anticancer, anti-leishmania, anti-inflammatory,
anti-allergic, immunomodulatory and
antiviral. The present study elaborates on the isolation, structural and functional characterization of Arbortristoside-C and its inhibition properties against
alpha-glucosidase, an important target for
diabetes mellitus. Arbortristoside-C is purified from seeds of N. arbor-tristis by extraction using polar fractionation and chromatographic techniques. Arbortristoside-C has been characterized using Ultra Violet (UV), Mass (MS), Infra-Red (IR) and Nuclear Magnetic Resonance (NMR). Inhibition kinetics and Isothermal Titration Calorimetry (ITC) were used for activity and binding characteristics of
acarbose and Arbortristoside-C using in-house purified α-
glucosidase from Bos taurus. Modeling, docking and structural comparison with
acarbose bound structure revealing the similar binding characteristics of Arbortristoside-C which include interaction with catalytic
acid/base
Aspartic acid residue. Cytotoxicity assay revealed that 100 µg/ml is the maximum toxic-free concentration of Arbortristoside-C. The purified Arbortristoside-C showed inhibition against mammalian α-
glucosidase, suggesting its potential to treat
Diabetes mellitus.