Accelerated Green Process of 2,5-Dimethylpyrazine Production from Glucose by Genetically Modified Escherichia coli.

Abstract

2,5-Dimethylpyrazine (2,5-DMP) is an indispensable additive for flavoring in the food industry and an important substrate for producing hypoglycemic and antilipolytic drugs. However, 2,5-DMP is produced by chemical synthesis in industry. Herein, a "green" strategy to produce 2,5-DMP has been reported for the first time. To do this, we rewrote the de novo 2,5-DMP biosynthesis pathway and substrate transmembrane transport in an l-threonine high-yielding strain to promote highly efficient 2,5-DMP production from glucose by submerged fermentation. The final strain T6-47-7 could produce 1.43 ± 0.07 g/L of 2,5-DMP with a carbon yield of 6.78% and productivity of 0.715 g/(L·d) in shake-flask fermentation using a phase-wise manner of hypoxia-inducible expression. The design-based strategy for constructing the 2,5-DMP high-yielding strain reported here could serve as a general concept for breeding high-yielding strains that produce some other type of alkylpyrazine.

• Authors: Jianzhong Xu, Haibo Yu, Xiulai Chen, Liming Liu, Weiguo Zhang
• Journal: ACS synthetic biology (ACS Synth Biol) Vol. 9 Issue 9 Pg. 2576-2587 (09 18 2020) ISSN: 2161-5063 [Electronic] United States
• PMID: 32841563 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Pyrazines
• NAD
• Alcohol Oxidoreductases
• L-threonine 3-dehydrogenase
• Oxidoreductases Acting on CH-NH Group Donors
• polyamine oxidase
• Glucose
• 2,5-dimethylpyrazine

Topics

• Alcohol Oxidoreductases (classification, genetics, metabolism)
• Biosynthetic Pathways (genetics)
• Escherichia coli (chemistry, metabolism)
• Glucose (metabolism)
• Metabolic Engineering (methods)
• NAD (chemistry, metabolism)
• Oxidoreductases Acting on CH-NH Group Donors (genetics)
• Phylogeny
• Pyrazines (chemistry, metabolism)
• Streptococcus (enzymology)