A Novel Anti-inflammatory Phenotype Transformed by Repetitive Low-dose Lipopolysaccharide in Primary Peritoneal Tissue-resident Macrophages.

Abstract

BACKGROUND/AIM:

Our previous studies suggested that oral administration of lipopolysaccharide (LPS) regulates the progression of various diseases via transformation of tissue-resident macrophages (MΦ). Recently, we characterized microglia transformed by repetitive low-dose LPS treatment (REPELL-microglia) in vitro, and this response was similar to that observed in response to oral administration of LPS in vivo. Here, we examined the characteristics of peritoneal tissue-resident MΦ (pMΦ) transformed by repetitive low-dose LPS treatment (REPELL-pMΦ).

MATERIALS AND METHODS:

Primary pMΦ were treated with low-dose LPS (1 ng/ml) three times; subsequently, phagocytic activity and gene expression were evaluated.

RESULTS:

REPELL-pMΦ exhibited high phagocytic activity and elevated expression of Arg1, Gipr, Gdnf, and Fpr2. The gene expression profiles observed in REPELL-pMΦ were distinct from those of REPELL-microglia.

CONCLUSION:

REPELL-pMΦ have the potential to promote clearance of xenobiotics and to suppress inflammation. The present study also demonstrates the diversity of tissue-resident MΦ transformation that reflect their tissue origin.

• Authors: Haruka Mizobuchi, Kazushi Yamamoto, Masafumi Yamashita, Hiroyuki Inagawa, Chie Kohchi, Gen-Ichiro Soma
• Journal: Anticancer research (Anticancer Res) Vol. 40 Issue 8 Pg. 4457-4464 (Aug 2020) ISSN: 1791-7530 [Electronic] Greece
• PMID: 32727775 (Publication Type: Journal Article)
• Copyright: Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Chemical References

• Gdnf protein, mouse
• Glial Cell Line-Derived Neurotrophic Factor
• Lipopolysaccharides
• Receptors, Formyl Peptide
• Receptors, Gastrointestinal Hormone
• formyl peptide receptor 2, mouse
• gastric inhibitory polypeptide receptor
• Arg1 protein, mouse
• Arginase

Topics

• Administration, Oral
• Animals
• Arginase (genetics)
• Gene Expression Profiling (methods)
• Gene Expression Regulation (drug effects)
• Glial Cell Line-Derived Neurotrophic Factor (genetics)
• Lipopolysaccharides (administration & dosage, adverse effects)
• Macrophages, Peritoneal (cytology, drug effects, physiology)
• Male
• Mice
• Organ Specificity
• Phagocytosis (drug effects)
• Phenotype
• Primary Cell Culture
• Receptors, Formyl Peptide (genetics)
• Receptors, Gastrointestinal Hormone (genetics)
• Up-Regulation