Abstract | BACKGROUND: METHODS: The theoretic interaction of piperlonguminine (PPLG) with ALDH2 was evaluated by docking analysis. Recombinant human ALDH2 was used to evaluate the effects of PPLG on the kinetics of the enzyme. The effects of PPLG were further investigated in a myocardial infarction model in rats, evaluating ALDHs activity, antioxidant enzymes, oxidative stress markers and mitochondrial function. RESULTS: PPLG increased the activity of recombinant human ALDH2 and protected the enzyme from inactivation by lipid aldehydes. Additionally, administration of this drug prevented the damage induced by ischemia/reperfusion in rats, restoring heart rate and blood pressure, which correlated with protection of ALDHs activity in the tissue, a lower content of lipid aldehydes, and the preservation of mitochondrial function. CONCLUSION:
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Authors | Belem Yoval-Sánchez, Luis Francisco Calleja, María de la Luz Hernández-Esquivel, José Salud Rodríguez-Zavala |
Journal | Biochimica et biophysica acta. General subjects
(Biochim Biophys Acta Gen Subj)
Vol. 1864
Issue 11
Pg. 129684
(11 2020)
ISSN: 1872-8006 [Electronic] Netherlands |
PMID | 32679250
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2020 Elsevier B.V. All rights reserved. |
Chemical References |
- Cardiotonic Agents
- Dioxolanes
- Aldehyde Dehydrogenase, Mitochondrial
- piperlonguminine
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Topics |
- Aldehyde Dehydrogenase, Mitochondrial
(metabolism)
- Animals
- Cardiotonic Agents
(therapeutic use)
- Dioxolanes
(therapeutic use)
- Enzyme Activation
(drug effects)
- Heart Rate
(drug effects)
- Humans
- Male
- Myocardial Infarction
(metabolism, physiopathology, prevention & control)
- Myocardial Reperfusion Injury
(metabolism, physiopathology, prevention & control)
- Rats, Wistar
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