Abstract |
Acute spinal cord injury (SCI) induces severe neuroinflammation, which increases intermediary filaments and neurodegeneration. Previous studies have shown that a basic fibroblast growth factor (bFGF) and dental pulp stem cells (DPSCs) contribute to a protective effect on injured neuronal cells, but the mechanism of SCI repair is still unclear. In this study, in situ heparin (HeP) hydrogel injection containing bFGF and DPSCs (HeP-bFGF-DPSCs), as well as in vitro studies of bFGF and DPSCs, proved an effective control over inflammation. The in vivo application of HeP-bFGF-DPSCs regulated inflammatory reactions and accelerated the nerve regeneration through microtubule stabilization and tissue vasculature. Our mechanistic investigation also showed that bFGF-DPSCs treatment inhibited microglia/macrophage proliferation and activation. Furthermore, HeP-bFGF-DPSCs prevented microglia/macrophage activation and reduced proinflammatory cytokine release. In this paper, we discovered that bFGF and DPSCs worked together to attenuate tissue inflammation of the injured spinal cord, resulting in a superior nerve repair. Our results indicated that a thermosensitive hydrogel delivering bFGF and DPSCs could serve as a promising treatment option for spinal cord injuries.
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Authors | Abdullkhaleg Albashari, Yan He, Yanni Zhang, Jihea Ali, Feiou Lin, Zengming Zheng, Keke Zhang, Yanfan Cao, Chun Xu, Lihua Luo, Jianming Wang, Qingsong Ye |
Journal | ACS omega
(ACS Omega)
Vol. 5
Issue 26
Pg. 16064-16075
(Jul 07 2020)
ISSN: 2470-1343 [Electronic] United States |
PMID | 32656428
(Publication Type: Journal Article)
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Copyright | Copyright © 2020 American Chemical Society. |