Field application of a colorimetric method of assaying chloroquine and desethylchloroquine in urine.

Abstract

In a study in western Kenya of malaria-infected adult women who had been treated with chloroquine, we compared the level of chloroquine and its principal metabolite, desethylchloroquine, in urine, measured using a newly developed modified Haskins test, with the level of chloroquine in whole blood, determined by high-performance liquid chromatography. Over a 28-day follow-up period, 277 matched urine and blood samples from 81 women were evaluated. A high correlation was observed between the level of chloroquine in whole blood (in mug/l) and that of chloroquine + desethylchloroquine in urine (in mg/l). The test was easily performed and may be useful for monitoring use of chloroquine in a community and determining pre-study or post-treatment ingestion or absorption of the drug in in vivo studies of parasite sensitivity.

• Authors: R W Steketee, D L Mount, L C Patchen, S B Williams, F C Churchill, J M Roberts, D C Kaseje, A D Brandling-Bennett
• Journal: Bulletin of the World Health Organization (Bull World Health Organ) Vol. 66 Issue 4 Pg. 485-90 ( 1988) ISSN: 0042-9686 [Print] Switzerland
• PMID: 3262448 (Publication Type: Journal Article)

Chemical References

• Chloroquine
• desethylchloroquine

Topics

• Adolescent
• Chloroquine (analogs & derivatives, blood, urine)
• Chromatography, High Pressure Liquid
• Colorimetry (methods)
• Female
• Humans