Abstract |
A novel anti-B cell monoclonal antibody UCHB1 is described which detects an antigen present on a selected subpopulation of both normal and leukemic B cells. Approximately 20% of normal tonsil and peripheral blood (PB) B cells are UCHB 1+ and the majority of B cells from all cases of prolymphocytic leukemia (PLL) tested, together with a low, variable percentage of PB B cells from non-Hodgkin's lymphoma patients with centroblastic centrocytic leukemia also express this antigen. Chronic lymphocytic leukemia and hairy cell leukemia B cells, pre-B acute lymphoblastic leukemia and Epstein-Barr virus transformed cell lines all lack UCHB 1 positivity as do all non-B lineage leukocytes and cell lines so far tested. In tonsil sections UCHB 1 staining is almost completely confined to surface IgM+ mantle zone lymphocytes and follicular dendritic cells, but not B cells, within the germinal centers. UCHB 1 can induce a rise in the level of intracellular free calcium ([Ca2+]i) in PLL B cells and low concentrations of monoclonal antibody can result in the entry of these cells into cell cycle in the absence of additional factors. The proliferative effect of UCHB 1 is greatly enhanced in the presence of Staphylococcus aureus Cowan and, to a lesser extent, phorbol ester. A similar costimulatory effect is exerted on a proportion of normal tonsil B cells although here, despite inducing a rise in [Ca2+]i, UCHB 1 alone does not cause cells to proliferate. UCHB 1 may well prove to be a useful antibody both to distinguish between different B cell leukemias and to study the phenotype and function of leukemic and normal B cell subpopulations.
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Authors | R J Armitage, D J Rowe, P C Beverly |
Journal | European journal of immunology
(Eur J Immunol)
Vol. 18
Issue 1
Pg. 67-76
(Jan 1988)
ISSN: 0014-2980 [Print] Germany |
PMID | 3257923
(Publication Type: Journal Article)
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Chemical References |
- Antibodies, Monoclonal
- Antigens, Differentiation, B-Lymphocyte
- Receptors, Antigen, B-Cell
- Calcium
- Thymidine
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Topics |
- Animals
- Antibodies, Monoclonal
(physiology)
- Antigen-Antibody Reactions
- Antigens, Differentiation, B-Lymphocyte
(analysis, immunology)
- B-Lymphocytes
(classification, immunology, metabolism)
- Calcium
(metabolism)
- Cell Cycle
- Cell Line
- Humans
- Intracellular Fluid
(metabolism)
- Lymphocyte Activation
- Mice
- Mice, Inbred BALB C
- Palatine Tonsil
(cytology)
- Phenotype
- Receptors, Antigen, B-Cell
(analysis)
- Thymidine
(metabolism)
- Tumor Cells, Cultured
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