Abstract | INTRODUCTION: METHODS: HDPCs were exposed to either F. nucleatum or P. gingivalis or to the combination of both with an additional 30 minutes of 5 mmol/L adenosine triphosphate ( ATP) incubation for 24 hours. Escherichia coli lipopolysaccharide exposure was used as a control. Gene expression of NLRP3, AIM2, POP1, CARD16, TRIM16, ASC and caspase-1, and IL-1β were evaluated by reverse transcription polymerase chain reaction. The presence and levels of pro- and mature IL-1β were monitored by immunocytochemistry and the release with enzyme-linked immunosorbent assay. RESULTS: Up-regulation of NLRP3 and AIM2 was detected in all exposure groups. IL-1β was up-regulated in all groups, except for the F. nucleatum + ATP group. CARD16 was significantly down-regulated by F. nucleatum or P. gingivalis with or without ATP; however, POP1 was down-regulated only in P. gingivalis and E. coli LPS + ATP groups. P. gingivalis alone significantly increased intracellular pro- and mature IL-1β levels. CONCLUSIONS: P. gingivalis and F. nucleatum in the presence of ATP may play a significant role in IL-1β-induced pulpal inflammation by dysregulating inflammasomes and their regulators.
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Authors | Kübra Aral, Michael R Milward, Paul R Cooper |
Journal | Journal of endodontics
(J Endod)
Vol. 46
Issue 9
Pg. 1265-1272
(Sep 2020)
ISSN: 1878-3554 [Electronic] United States |
PMID | 32565332
(Publication Type: Journal Article)
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Copyright | Copyright © 2020 American Association of Endodontists. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- DNA-Binding Proteins
- Inflammasomes
- Interleukin-1beta
- NLR Family, Pyrin Domain-Containing 3 Protein
- Transcription Factors
- Tripartite Motif Proteins
- TRIM16 protein, human
- Ubiquitin-Protein Ligases
- Caspase 1
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Topics |
- Caspase 1
- DNA-Binding Proteins
- Dental Pulp
- Escherichia coli
- Fusobacterium nucleatum
- Humans
- Inflammasomes
- Interleukin-1beta
- NLR Family, Pyrin Domain-Containing 3 Protein
- Porphyromonas gingivalis
- Transcription Factors
- Tripartite Motif Proteins
- Ubiquitin-Protein Ligases
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