Abstract | BACKGROUND: METHODS: A mixed reference serum from three NPC patients diluted with healthy subject serum was made as a potential first international standard for VCA- IgA. VCA- IgA was detected in twenty NPC patients by four ELISA kits and two chemiluminescent immunoassays kits using the reference as a calibration curve. The performance of these six kits was evaluated, and the quantitative results were compared. RESULTS: Our results showed a good linearity of the reference in different kits. Without reference, the difference of the total coefficient of variation (from 3.98 to 43.11%) and Within-run coefficient of variation (from 2.47 to 19.66%) was large in the 6 kits. The positive and negative coincidence rate between the 6 kits and indirect immunofluorescence for NPC diagnosis was 75% overall agreement, but a difference among the six kits was found, ranging from 55 to 90%. The concentration of VCA- IgA in the 20 NPC samples led in the division into three categories such as negative, low, or medium/high positive, but these concentrations were significantly different within these three categories depending on the kit used of the 6 considered. However,a good correlation (R2 = 0.986) was observed between Antu and Beier ELISA kits. CONCLUSIONS: The reference serum mightbe used as a reference standard for a better comparison of the results from different kits/laboratories. However, the quantitative results of some kits are still inconsistent due to the diversity of VCA antigens.
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Authors | Hao Chen, Qiaohua Zhong, Xiaobin Wu, Yanling Ding, Qi Chen, Ning Xue, Yiwei Xu, Shulin Chen |
Journal | Infectious agents and cancer
(Infect Agent Cancer)
Vol. 15
Pg. 25
( 2020)
ISSN: 1750-9378 [Print] England |
PMID | 32368250
(Publication Type: Journal Article)
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Copyright | © The Author(s) 2020. |