Multienzyme detection and in-situ monitoring of enzyme activity by bending CE using quantum dots-based polypeptide substrate.

Abstract

Multienzyme detection and monitoring enzyme activity in situ are significant for the disease to diagnose. This study aims to develop a quantum dots (QDs)-based nanoprobe Cyanine5-DDDLEVLFQFPGLVPRGSGGHHHHHH-QDs (Cy5-LEVLVP-QD), which is able to detect two enzymes inside a bent capillary using CE. Cy5-LEVLVP and QDs were allowed to bind with each other through metal affinity interaction and then injected the Cy5-LEVLVP-QD complex into a capillary with different bends, followed by related enzyme that can cleave the Cy5-LEVLVP peptide. The fluorescence of Cy5 was excited by QDs due to Förster resonance energy transfer. By monitoring the peaks produced by the original Cy5-LEVLVP-QD complex and a significant fluorescence change, sensitive analysis of two different enzymes was conducted. Therefore, the novel approach of using capillaries with semicircular bends could prove particularly useful for enzyme investigating in disease.

• Authors: Lin Qiu, Pengfei Cui, Zhilan Zhu, Mingyu Xu, Wenjing Jia, Jingyu Sheng, Xinye Ni, Shuwen Zhou, Jianhao Wang
• Journal: Electrophoresis (Electrophoresis) Vol. 41 Issue 12 Pg. 1103-1108 (06 2020) ISSN: 1522-2683 [Electronic] Germany
• PMID: 32091140 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Carbocyanines
• Enzymes
• Fluorescent Dyes
• Peptides
• cyanine dye 5

Topics

• Carbocyanines (analysis, chemistry)
• Electrophoresis, Capillary (methods)
• Enzyme Assays
• Enzymes (analysis, metabolism)
• Fluorescence Resonance Energy Transfer (methods)
• Fluorescent Dyes (analysis, chemistry)
• Peptides (analysis, chemistry, metabolism)
• Quantum Dots (analysis, chemistry)