Systematic Analysis of the DNA Methylase and Demethylase Gene Families in Rapeseed (Brassica napus L.) and Their Expression Variations After Salt and Heat stresses.

Abstract	DNA methylation is a process through which methyl groups are added to the DNA molecule, thereby modifying the activity of a DNA segment without changing the sequence. Increasing evidence has shown that DNA methylation is involved in various aspects of plant growth and development via a number of key processes including genomic imprinting and repression of transposable elements. DNA methylase and demethylase are two crucial enzymes that play significant roles in dynamically maintaining genome DNA methylation status in plants. In this work, 22 DNA methylase genes and six DNA demethylase genes were identified in rapeseed (Brassica napus L.) genome. These DNA methylase and DNA demethylase genes can be classified into four (BnaCMTs, BnaMET1s, BnaDRMs and BnaDNMT2s) and three (BnaDMEs, BnaDML3s and BnaROS1s) subfamilies, respectively. Further analysis of gene structure and conserved domains showed that each sub-class is highly conserved between rapeseed and Arabidopsis. Expression analysis conducted by RNA-seq as well as qRT-PCR suggested that these DNA methylation/demethylation-related genes may be involved in the heat/salt stress responses in rapeseed. Taken together, our findings may provide valuable information for future functional characterization of these two types of epigenetic regulatory enzymes in polyploid species such as rapeseed, as well as for analyzing their evolutionary relationships within the plant kingdom.
Authors	Shihang Fan, Hongfang Liu, Jing Liu, Wei Hua, Shouming Xu, Jun Li
Journal	International journal of molecular sciences (Int J Mol Sci) Vol. 21 Issue 3 (Jan 31 2020) ISSN: 1422-0067 [Electronic] Switzerland
PMID	32023925 (Publication Type: Journal Article)
Chemical References	Plant Proteins DNA Modification Methylases
Topics	Brassica napus (genetics, growth & development, metabolism) DNA Methylation DNA Modification Methylases (chemistry, genetics, metabolism) Epigenesis, Genetic Gene Expression Profiling (methods) Gene Expression Regulation, Plant Heat-Shock Response Multigene Family Phylogeny Plant Proteins (chemistry, genetics, metabolism) Protein Domains Salt Stress Sequence Analysis, RNA Tissue Distribution

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