Iron-induced derangement in hepatic Δ-5 and Δ-6 desaturation capacity and fatty acid profile leading to steatosis: Impact on extrahepatic tissues and prevention by antioxidant-rich extra virgin olive oil.

Abstract

The administration of iron induces liver oxidative stress and depletion of long-chain polyunsaturated fatty acids (LCPUFAs), n-6/n-3 LCPUFA ratio enhancement and fat accumulation, which may be prevented by antioxidant-rich extra virgin olive oil (AR-EVOO) supplementation. Male Wistar rats were subjected to a control diet (50 mg iron/kg diet) or iron-rich diet (IRD; 200 mg/kg diet) with alternate AR-EVOO for 21 days. Liver fatty acid (FA) analysis was performed by gas-liquid chromatography (GLC) after lipid extraction and fractionation, besides Δ-5 desaturase (Δ-5 D) and Δ6-D mRNA expression (qPCR) and activity (GLC) measurements. The IRD significantly (p < 0.05) increased hepatic total fat, triacylglycerols, free FA contents and serum transaminases levels, with diminution in those of n-6 and n-3 LCPUFAs, higher n-6/n-3 ratios, lower unsaturation index and Δ5-D and Δ6-D activities, whereas the mRNA expression of both desaturases was enhanced over control values, changes that were prevented by concomitant AR-EVOO supplementation. N-6 and n-3 LCPUFAs were also decreased by IRD in extrahepatic tissues and normalized by AR-EVOO. In conclusion, AR-EVOO supplementation prevents IRD-induced changes in parameters related to liver FA metabolism and steatosis, an effect that may have a significant impact in the treatment of iron-related pathologies or metabolic disorders such as non-alcoholic fatty liver disease.

• Authors: Cynthia Barrera, Rodrigo Valenzuela, Miguel A Rincón, Alejandra Espinosa, Sandra López-Arana, Daniel González-Mañan, Nalda Romero, Romina Vargas, Luis A Videla
• Journal: Prostaglandins, leukotrienes, and essential fatty acids (Prostaglandins Leukot Essent Fatty Acids) Vol. 153 Pg. 102058 (02 2020) ISSN: 1532-2823 [Electronic] Scotland
• PMID: 32007744 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2020 Elsevier Ltd. All rights reserved.

Chemical References

• Antioxidants
• Delta-5 Fatty Acid Desaturase
• Fatty Acids
• Olive Oil
• Iron
• Fatty Acid Desaturases
• Linoleoyl-CoA Desaturase

Topics

• Animals
• Antioxidants (administration & dosage, chemistry, pharmacology)
• Chromatography, Gas
• Delta-5 Fatty Acid Desaturase
• Disease Models, Animal
• Fatty Acid Desaturases (genetics)
• Fatty Acids (analysis)
• Fatty Liver (chemically induced, epidemiology, prevention & control)
• Gene Expression Regulation, Enzymologic (drug effects)
• Iron (adverse effects)
• Linoleoyl-CoA Desaturase (genetics)
• Male
• Olive Oil (administration & dosage, chemistry, pharmacology)
• Rats
• Rats, Wistar