Effect of Sorbus domestica and its active constituents in an experimental model of colitis rats induced by acetic acid.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

In Turkish folk medicine, leaves of Sorbus domestica are used for the treatment of burns, cough, stomachache, bradyuria, kidney stone. The fruits of this plant are used for diarrhoea.

AIM OF THE STUDY:

This study was carried out to investigate the effect of S. domestica on ulcerative colitis induced by acetic acid in rats.

MATERIALS AND METHODS:

The crude methanolic extract of fruits was sequentially fractionated into five subextracts; dichloromethane, diethyl ether, ethyl acetate, n-butanol and aqueous extracts. Effects of the extract, subextracts and fractions were investigated in acetic acid-induced rat colitis model. The colonic interleukin-6 (IL-6), tumor necrosis factor (TNF-α), nitrite, superoxide dismutase (SOD), glutathione (GSH), lipid peroxidation (LPO), catalase (CAT), and malondialdehyde (MDA) levels as well as the caspase-3 and myeloperoxidase (MPO) activities were measured to determine the activity. Histopathological analyzes were also performed on the colon tissue of rats.

RESULTS:

The methanolic extract and diethylether subextract have led to a noteworthy decrease in MPO, caspase-3, IL-6, TNF-α, MDA, and nitrite levels in the colon tissue and blood. In addition, histopathological analysis results were supported by biochemical parameters. After confirmation of the activity against ulcerative colitis, the diethyl ether subextract was subjected to more chromatographic separation for the isolation of compounds 1, 2 and 3. The structures of these three compounds were elucidated as vanillic acid 4-O-α-L-rhamnopyranoside (1), protocateuic acid anhydrite (2) and trivanilloyl-(1,3,4-trihydroxybenzol) ester (3).

CONCLUSION:

In this study, the potential of S. domestica in the treatment of colitis was investigated. Fruits of this plant were found to have important anti-inflammatory and antioxidant activities. Through isolation techniques, vanillic acid 4-O-α-L-rhamnopyranoside, protocateuic acid anhydrite and trivanilloyl-(1,3,4-trihydroxybenzol) ester were determined as the main active components of the fruits. Consequently, S domestica might be a promising candidate for upcoming use the prevention and treatment of various disorders, such as inflammatory bowel diseases, irritable bowel syndrome and Clostridium difficile infection.

• Authors: Esra Küpeli Akkol, Fatma Tuğçe Gürağaç Dereli, Hakkı Taştan, Eduardo Sobarzo-Sánchez, Haroon Khan
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 251 Pg. 112521 (Apr 06 2020) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 31883473 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Elsevier B.V. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Antioxidants
• Il6 protein, rat
• Interleukin-6
• Nitrites
• Plant Extracts
• Tumor Necrosis Factor-alpha
• Malondialdehyde
• Catalase
• Peroxidase
• Superoxide Dismutase
• Glutathione
• Acetic Acid

Topics

• Acetic Acid
• Animals
• Anti-Inflammatory Agents (pharmacology, therapeutic use)
• Antioxidants (pharmacology, therapeutic use)
• Catalase (metabolism)
• Colitis, Ulcerative (chemically induced, drug therapy, metabolism, pathology)
• Colon (drug effects, metabolism, pathology)
• Disease Models, Animal
• Fruit
• Glutathione (metabolism)
• Interleukin-6 (metabolism)
• Lipid Peroxidation (drug effects)
• Male
• Malondialdehyde (metabolism)
• Nitrites (metabolism)
• Peroxidase (metabolism)
• Plant Extracts (pharmacology, therapeutic use)
• Rats, Sprague-Dawley
• Sorbus
• Superoxide Dismutase (metabolism)
• Tumor Necrosis Factor-alpha (metabolism)