Development of a yeast model to study the contribution of vacuolar polyphosphate metabolism to lysine polyphosphorylation.

Abstract

A recently-discovered protein post-translational modification, lysine polyphosphorylation (K-PPn), consists of the covalent attachment of inorganic polyphosphate (polyP) to lysine residues. The nonenzymatic nature of K-PPn means that the degree of this modification depends on both polyP abundance and the amino acids surrounding the modified lysine. K-PPn was originally discovered in budding yeast (Saccharomyces cerevisiae), in which polyP anabolism and catabolism are well-characterized. However, yeast vacuoles accumulate large amounts of polyP, and upon cell lysis, the release of the vacuolar polyP could nonphysiologically cause K-PPn of nuclear and cytosolic targets. Moreover, yeast vacuoles possess two very active endopolyphosphatases, Ppn1 and Ppn2, that could have opposing effects on the extent of K-PPn. Here, we characterized the contribution of vacuolar polyP metabolism to K-PPn of two yeast proteins, Top1 (DNA topoisomerase 1) and Nsr1 (nuclear signal recognition 1). We discovered that whereas Top1-targeting K-PPn is only marginally affected by vacuolar polyP metabolism, Nsr1-targeting K-PPn is highly sensitive to the release of polyP and of endopolyphosphatases from the vacuole. Therefore, to better study K-PPn of cytosolic and nuclear targets, we constructed a yeast strain devoid of vacuolar polyP by targeting the exopolyphosphatase Ppx1 to the vacuole and concomitantly depleting the two endopolyphosphatases (ppn1Δppn2Δ, vt-Ppx1). This strain enabled us to study K-PPn of cytosolic and nuclear targets without the interfering effects of cell lysis on vacuole polyP and of endopolyphosphatases. Furthermore, we also define the fundamental nature of the acidic amino acid residues to the K-PPn target domain.

• Authors: Cristina Azevedo, Yann Desfougères, Yannasittha Jiramongkol, Hamish Partington, Sasanan Trakansuebkul, Jyoti Singh, Nicole Steck, Henning J Jessen, Adolfo Saiardi
• Journal: The Journal of biological chemistry (J Biol Chem) Vol. 295 Issue 6 Pg. 1439-1451 (02 07 2020) ISSN: 1083-351X [Electronic] United States
• PMID: 31844018 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2020 Azevedo et al.

Chemical References

• NSR1 protein, S cerevisiae
• Nuclear Proteins
• Polyphosphates
• RNA-Binding Proteins
• Saccharomyces cerevisiae Proteins
• TOP1 protein, S cerevisiae
• DNA Topoisomerases, Type I
• Lysine

Topics

• DNA Topoisomerases, Type I (metabolism)
• Lysine (metabolism)
• Nuclear Proteins (metabolism)
• Phosphorylation
• Polyphosphates (metabolism)
• Protein Processing, Post-Translational
• RNA-Binding Proteins (metabolism)
• Saccharomyces cerevisiae (metabolism)
• Saccharomyces cerevisiae Proteins (metabolism)
• Vacuoles (metabolism)