The histone methyltransferase KMT2D, mutated in Kabuki syndrome patients, is required for neural crest cell formation and migration.

Abstract

Kabuki syndrome is an autosomal dominant developmental disorder with high similarities to CHARGE syndrome. It is characterized by a typical facial gestalt in combination with short stature, intellectual disability, skeletal findings and additional features like cardiac and urogenital malformations, cleft palate, hearing loss and ophthalmological anomalies. The major cause of Kabuki syndrome are mutations in KMT2D, a gene encoding a histone H3 lysine 4 (H3K4) methyltransferase belonging to the group of chromatin modifiers. Here we provide evidence that Kabuki syndrome is a neurocrestopathy, by showing that Kmt2d loss-of-function inhibits specific steps of neural crest (NC) development. Using the Xenopus model system, we find that Kmt2d loss-of-function recapitulates major features of Kabuki syndrome including severe craniofacial malformations. A detailed marker analysis revealed defects in NC formation as well as migration. Transplantation experiments confirm that Kmt2d function is required in NC cells. Furthermore, analyzing in vivo and in vitro NC migration behavior demonstrates that Kmt2d is necessary for cell dispersion but not protrusion formation of migrating NC cells. Importantly, Kmt2d knockdown correlates with a decrease in H3K4 monomethylation and H3K27 acetylation supporting a role of Kmt2d in the transcriptional activation of target genes. Consistently, using a candidate approach, we find that Kmt2d loss-of-function inhibits Xenopus Sema3F expression, and overexpression of Sema3F can partially rescue Kmt2d loss-of-function defects. Taken together, our data reveal novel functions of Kmt2d in multiple steps of NC development and support the hypothesis that major features of Kabuki syndrome are caused by defects in NC development.

• Authors: Janina Schwenty-Lara, Denise Nehl, Annette Borchers
• Journal: Human molecular genetics (Hum Mol Genet) Vol. 29 Issue 2 Pg. 305-319 (01 15 2020) ISSN: 1460-2083 [Electronic] England
• PMID: 31813957 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © The Author(s) 2019. Published by Oxford University Press.

Chemical References

• DNA-Binding Proteins
• Histones
• KMT2D protein, human
• Neoplasm Proteins
• Semaphorins
• Xenopus Proteins
• Histone-Lysine N-Methyltransferase
• kmt2d protein, Xenopus

Topics

• Abnormalities, Multiple (enzymology, genetics, metabolism, pathology)
• Acetylation
• Animals
• Cell Movement (genetics)
• DNA-Binding Proteins (genetics, metabolism)
• Face (abnormalities, pathology)
• Hematologic Diseases (enzymology, genetics, metabolism, pathology)
• Histone-Lysine N-Methyltransferase (genetics, metabolism)
• Histones (metabolism)
• Loss of Function Mutation
• Methylation
• Mutation
• Neoplasm Proteins (genetics, metabolism)
• Neural Crest (enzymology, metabolism, pathology)
• Neural Plate (growth & development, metabolism, pathology)
• Semaphorins (genetics, metabolism)
• Vestibular Diseases (enzymology, genetics, metabolism, pathology)
• Xenopus (embryology, genetics, metabolism)
• Xenopus Proteins (genetics, metabolism, physiology)