Abstract |
We designed a novel luminescent metal-organic framework (MOF) named Ce- TCPP-LMOF (CTM) through a simple one-pot solvothermal method. CTM was synthesized by using the emerging electrochemiluminescent (ECL) material (4,4',4″,4‴-( porphine-5,10,15,20-tetrayl)tetrakis( benzoic acid) as the organic ligand and Ce(III) as the metal node. We found that CTM not only has the remarkable ability to emit light but also has a uniform "sandwich biscuit" shape and suitable nanoscale size, which are promising for further applications. We also applied CTM to construct a novel ECL immunosensor and achieve sensitive detection of the proprotein convertase subtilisin/kexin type 9 (PCSK9), a biomarker related to cardiovascular diseases. To further amplify the ECL signal of CTM, a novel dual-amplified signal strategy was established by inducing a polyamidoamine dendrimer (PAMAM) and gold nanoparticles (AuNPs). Importantly, we first proved that the ECL signal of the CTM/S2O82- system could be enhanced by the PAMAM electric field. As the electron transfer rate was accelerated by the AuNP layer, this ECL signal was further enhanced in AuNP-modified electrodes. The ECL immunosensor showed desirable performance for PCSK9 analysis within a detection range of 50 fg mL-1 to 10 ng mL-1 and a low limit of detection of 19.12 ± 2.69 fg mL-1. Real sample detection suggested that the immunosensor holds great potential for analyzing clinical serum samples.
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Authors | Yuan Zhou, Junlin He, Chengli Zhang, Jia Li, Xiaoxue Fu, Weiran Mao, Wenming Li, Chao Yu |
Journal | ACS applied materials & interfaces
(ACS Appl Mater Interfaces)
Vol. 12
Issue 1
Pg. 338-346
(Jan 08 2020)
ISSN: 1944-8252 [Electronic] United States |
PMID | 31794188
(Publication Type: Journal Article)
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Chemical References |
- Dendrimers
- Metal-Organic Frameworks
- Poly(amidoamine)
- Polyamines
- PCSK9 protein, human
- Proprotein Convertase 9
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Topics |
- Biosensing Techniques
(methods)
- Dendrimers
(chemistry)
- Electrochemical Techniques
(methods)
- Immunoassay
(methods)
- Luminescence
- Luminescent Measurements
(methods)
- Metal-Organic Frameworks
(chemistry)
- Microscopy, Electron, Scanning
- Polyamines
(chemistry)
- Proprotein Convertase 9
(chemistry)
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