Cryptotanshinone attenuates allergic airway inflammation through negative regulation of NF-κB and p38 MAPK.

Abstract

This study is to determine the role and mechanism of cryptotanshinone (CTS) in allergic airway inflammation. Asthma induced by OVA was established in BALB/c mice. We found increased airway hyperresponsiveness (AHR), increased inflammatory cell infiltration, elevated levels of TNF-α, interleukin-1β (IL-1β), IL-4, IL-5, IL-6 and IL-13, decreased interferon gamma (IFN-γ) in lung tissue, increased content of total immunoglobulin E (IgE), OVA specific IgE, Eotaxin, ICAM-1, VCAM-1, nuclear factor-kappaB (NF-κB) and phosphorylation of p38 MAPK in lung tissue. However, the administration of CTS significantly decreased AHR in asthmatic mice, reduced inflammation around the bronchioles and inflammatory cells around airway, regulated cytokine production, reduced the total IgE and OVA-specific IgE levels, and inhibited NF-κB activation and p38 MAPK phosphorylation. In vitro experiments in 16 HBE cells revealed that CTS attenuated CAM-1 and IL-6 expression. These results indicate that CTS alleviates allergic airway inflammation by modulating p38 MAPK phosphorylation and NF-κB activation.

• Authors: Junfeng Li, Mingyu Zheng, Chongyang Wang, Jingzhi Jiang, Chang Xu, Li Li, Liangchang Li, Guanghai Yan, Yongde Jin
• Journal: Bioscience, biotechnology, and biochemistry (Biosci Biotechnol Biochem) Vol. 84 Issue 2 Pg. 268-278 (Feb 2020) ISSN: 1347-6947 [Electronic] England
• PMID: 31690224 (Publication Type: Journal Article)

Chemical References

• Cytokines
• Drugs, Chinese Herbal
• NF-kappa B
• Phenanthrenes
• Immunoglobulin E
• cryptotanshinone
• Ovalbumin
• p38 Mitogen-Activated Protein Kinases

Topics

• Animals
• Asthma (metabolism, pathology)
• Bronchial Hyperreactivity
• Bronchoalveolar Lavage Fluid (cytology)
• Chemotaxis, Leukocyte (drug effects)
• Cytokines (metabolism)
• Drugs, Chinese Herbal
• Female
• Hypersensitivity (metabolism, pathology)
• Immunoglobulin E (metabolism)
• Inflammation (metabolism, pathology)
• Lung (metabolism)
• Mice
• Mice, Inbred BALB C
• NF-kappa B (metabolism)
• Ovalbumin (toxicity)
• Phenanthrenes (pharmacology)
• Phosphorylation
• p38 Mitogen-Activated Protein Kinases (metabolism)