Abstract | BACKGROUND: Natural extracts with beneficial biological activities are nowadays of high interest, in various treatment or prophylaxis. Hypericum capitatum has been known for its curative effects for centuries and its extracts have become of interest due to their distinct activity among other Hypericaceae members. In this study, further light is aimed to be shed on the secondary-metabolites composition of H. capitatum extracts, using chromatographic techniques and Electron paramagnetic resonance profiles in alkaline medium. Considering that no previous works explored the anti-inflammatory activity of H. capitatum, here, an in vivo study is also designed in order to evaluate this property by assessing the impact of one of H. capitatum extracts in ameliorating turpentine oil-induced inflammation on rats and to quantify their blood antioxidants level. METHODS: RESULTS: The extract was remarkably rich in rutin; however, other polyphenolic-like minor components appeared important in explaining the observed biological properties. The tested extract prevents the increase of inflammation-induced white blood cell count, number of neutrophils, and serum nitric oxide, and did so in a dose-dependent manner, similarly to the positive control- diclofenac. In addition, the same extract appeared to be a good alternative to diclofenac to restore total oxidative status, thiobarbituric active reactive species, total proteins and C reactive proteins. Moreover, antioxidant enzymes such as catalase, superoxide dismutase and total serum thiol concentration were significantly increased by the tested extract. CONCLUSIONS: Due to its powerful reservoir rich in rutin, H. capitatum extract depicted its in vivo antioxidant and anti-inflammatory effects indicating it to be a good alternative to conventional drugs for oxidative stress protection.
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Authors | Anca D Farcas, Augustin C Mot, Cezara Zagrean-Tuza, Madalina Ticolea, Bogdan Sevastre, Muhittin Kulak, Radu Silaghi-Dumitrescu, Alina Parvu |
Journal | BMC complementary and alternative medicine
(BMC Complement Altern Med)
Vol. 19
Issue 1
Pg. 289
(Oct 29 2019)
ISSN: 1472-6882 [Electronic] England |
PMID | 31664997
(Publication Type: Journal Article)
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Chemical References |
- Anti-Inflammatory Agents
- Plant Extracts
- Malondialdehyde
- Rutin
- Catalase
- Superoxide Dismutase
- Turpentine
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Topics |
- Animals
- Anti-Inflammatory Agents
(administration & dosage, chemistry)
- Catalase
(metabolism)
- Female
- Humans
- Hypericum
(chemistry)
- Inflammation
(chemically induced, drug therapy, metabolism)
- Male
- Malondialdehyde
(metabolism)
- Oxidative Stress
(drug effects)
- Plant Extracts
(administration & dosage, chemistry)
- Rats
- Rats, Wistar
- Rutin
(administration & dosage, analysis)
- Superoxide Dismutase
(metabolism)
- Turpentine
(adverse effects)
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