Inter- and Intra-assay Variation in the Diagnostic Performance of Assays for Anti-tissue Transglutaminase in 2 Populations.

Abstract

Tissue transglutaminse-2 (TG2)-based immunoassays are the cornerstone of diagnosis in celiac disease (CeD), with a reported pooled sensitivity as high as 98%.1 However, a few small, single-center studies have questioned their sensitivity in clinical practice.2-5 Moreover, commercial kits use variable TG2 antigens,6 with cutoffs determined by using small, poorly defined populations. Variation in diagnostic performance of anti-TG2 assays in different racial and geographic populations has not yet been studied. We compared the interassay and intra-assay variations in diagnostic performance of 4 immunoglobulin (Ig)A-anti-TG2 assays in Canadian and Indian populations.

• Authors: Prashant Singh, Alka Singh, Jocelyn A Silvester, Vikas Sachdeva, Xinhua Chen, Hua Xu, Daniel A Leffler, Vineet Ahuja, Donald R Duerksen, Ciaran P Kelly, Govind K Makharia
• Journal: Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association (Clin Gastroenterol Hepatol) Vol. 18 Issue 11 Pg. 2628-2630 (10 2020) ISSN: 1542-7714 [Electronic] United States
• PMID: 31546060 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: Copyright © 2020 AGA Institute. Published by Elsevier Inc. All rights reserved.

Chemical References

• Autoantibodies
• Immunoglobulin A
• Transglutaminases

Topics

• Autoantibodies
• Canada
• Celiac Disease (diagnosis)
• Humans
• Immunoassay
• Immunoglobulin A
• Transglutaminases