Acute pancreatitis (AP) is a severe inflammatory condition of the pancreas, with no specific treatment available. We have previously reported that Nardostachys jatamansi (NJ) ameliorates
cerulein-induced AP. However, the specific compound responsible for this inhibitory effect has not been identified. Therefore, in the present study, we focused on a single compound, 8α-hydroxypinoresinol (HP), from NJ. The aim of this study was to determine the effect of HP on the development of
pancreatitis in mice and to explore the underlying mechanism(s). AP was induced by the injection of
cerulein (50 μg/kg/h) for 6 h. HP (0.5, 5 or 10 mg/kg, i.p.) was administered 1 h prior to and 1, 3 or 5 h after the first
cerulein injection, with vehicle- and
DMSO-treated groups as controls. Blood samples were collected to determine serum levels of
amylase,
lipase, and
cytokines. The pancreas was removed for morphological examination,
myeloperoxidase (MPO) assays,
cytokine assays, and assessment of nuclear factor (NF)-κB activation. The lungs were removed for morphological examination and MPO assays. Administration of HP dramatically improved pancreatic damage and
pancreatitis-associated lung damage and also reduced
amylase and
lipase activities in serum. Moreover, administration of HP reduced the production of pro-inflammatory
cytokines, such as
tumor necrosis factor (TNF)-α,
interleukin (IL)-1β, and
IL-6 in the pancreas and serum during AP. In addition, the administration of HP inhibited degradation of inhibitory κ-Bα (Iκ-Bα), NF-κB p65 translocation into nucleus and NF-κB binding activity in the pancreas. Our results suggest that HP exerted
therapeutic effects on
pancreatitis and these beneficial effects may be due to the inhibition of NF-κB activation.