Abstract | PURPOSES: METHODS: Twenty mini-pigs with morbid obesity were fed an obesogenic diet enriched with LCS ( sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group), or without LCS (control group), for 3 months. Glucose uptake and insulin sensitivity were determined for the duodenum, liver, skeletal muscle, adipose tissue and brain using dynamic PET 18FDG scanning together with direct measurement of arterial input function. Body composition was also measured using CT imaging and energy metabolism quantified with indirect calorimetry. RESULTS: The LCS diet increased subcutaneous abdominal fat by ≈ 20% without causing weight gain, and reduced insulin clearance by ≈ 40%, while whole-body glucose uptake and insulin sensitivity were unchanged. In contrast, glucose uptake in the duodenum, liver and brain increased by 57, 66 and 29% relative to the control diet group (P < 0.05 for all), while insulin sensitivity increased by 53, 55 and 28% (P < 0.05 for all), respectively. In the brain, glucose uptake increased significantly only in the frontal cortex, associated with improved metabolic connectivity towards the hippocampus and the amygdala. CONCLUSIONS:
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Authors | Charles-Henri Malbert, Michael Horowitz, Richard L Young |
Journal | European journal of nuclear medicine and molecular imaging
(Eur J Nucl Med Mol Imaging)
Vol. 46
Issue 11
Pg. 2380-2391
(Oct 2019)
ISSN: 1619-7089 [Electronic] Germany |
PMID | 31338548
(Publication Type: Journal Article)
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Chemical References |
- Insulin
- Sweetening Agents
- Thiazines
- Fluorodeoxyglucose F18
- Sucrose
- trichlorosucrose
- Glucose
- acetosulfame
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Topics |
- Adipose Tissue
(metabolism)
- Amygdala
(diagnostic imaging)
- Animal Feed
- Animals
- Body Composition
- Disease Models, Animal
- Female
- Fluorodeoxyglucose F18
- Frontal Lobe
(diagnostic imaging)
- Glucose
(metabolism)
- Hippocampus
(diagnostic imaging)
- Insulin
(metabolism)
- Insulin Resistance
- Male
- Obesity
(metabolism, physiopathology)
- Sucrose
(analogs & derivatives, pharmacology)
- Sweetening Agents
(pharmacology)
- Swine
- Swine, Miniature
- Thiazines
(pharmacology)
- Tomography, X-Ray Computed
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